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| 杨树桑黄子实体总黄酮提取工艺优化及其生物活性评价 |
| Optimization of Extraction Process and Bioactivity Evaluation for Total Flavonoids from Sanghuangporus vaninii Fruiting Body |
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| DOI：doi:10.3969/j.issn.1005-7021.2026.03.006 |
| 中文关键词: 杨树桑黄（Sanghuangporus vaninii）子实体 总黄酮 提取优化 抗氧化活性 降血糖活性 |
| 英文关键词: Sanghuangporus vaninii fruiting body total flavonoids extraction optimization antioxidant activity hypoglycemic activity |
| 基金项目:辽宁省农业科学院协同创新专项（2024XTCX0403）；辽宁省科技厅应用基础研究计划项目（2025080179-JH2/1013） |
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| 摘要点击次数: 34 |
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| 中文摘要: |
| 为提高杨树桑黄（Sanghuangporus vaninii ）子实体总黄酮得率，并对其生物活性进行评价，以杨树桑黄子实体为原料，采用超声波辅助法提取其总黄酮，以总黄酮得率为考察指标，利用响应面法优化总黄酮提取工艺，测定其降血糖活性及抗氧化活性。结果表明，杨树桑黄总黄酮最佳提取工艺为以60%乙醇为提取剂，液料比 42∶1（mL/g）、提取时间2.7 h、提取温度75 ℃，总黄酮得率为（49.31±0.45） mg/g。体外抗氧化实验结果显示，杨树桑黄总黄酮具有较好的总抗氧化能力，其清除DPPH自由基、羟自由基的半抑制质量浓度（IC50）分别为（1.31±0.06）、（73.62±1.50） μg/mL。体外降血糖实验显示，杨树桑黄总黄酮抑制α-葡萄糖苷酶和α-淀粉酶活性的IC50分别为（0.042±0.002）、（0.324±0.083） μg/mL，抑制类型均为竞争性-非竞争性混合抑制。杨树桑黄总黄酮提取工艺简单、易行，提取的总黄酮具有较好的抗氧化活性和降血糖活性。 |
| 英文摘要: |
| In order to improve the yield of total flavonoids from fruiting body of Sanghuangporus vaninii, and to evaluate biological activity of the total flavonoids, the ultrasonic-assisted method was used to extract total flavonoids from raw material of S. vaninii fruiting body with the yield of total flavonoids as indicator, the extraction process was optimized using response surface methodology, and the hypoglycemic activity and antioxidant activity were determined. The results showed that the optimal extraction process ofS. vaninii total flavonoids was with 60％ethanol as solvent, liquid-solid ratio of 42∶1 mL/g , extraction time of 2.7 h, and extraction temperature of 75 ℃, and the yield of S. vaninii total flavonoids was （49.31±0.45） mg/g, and the total antioxidant capacity of S. vaninii total flavonoids through the experiments in vitro are good. The IC50 of S. vaninii total flavonoids for scavenging DPPH free radicals and hydroxyl free radicals were （1.31±0.06） μg/mL and （73.62±1.50） μg/mL, respectively. The results of hypoglycemic activity analysis in vitro showed that IC50 of S. vaninii total flavonoids for inhibiting α-glucosidase and α-amylase were （0.042±0.002）μg/mL and （0.324±0.083）μg/mL with both of competitive-noncompetitive mixed inhibition, respectively. The extraction process of S. vaninii total flavonoids is simple and feasible to conduct, and S. vaninii total flavonoids have good antioxidant and hypoglycemic activity. |
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