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| 塔里木西缘沙枣根际促生菌的筛选鉴定及促生效果研究 |
| The Screening, Identification, and Growth-Promoting Effect of Rhizosphere Bacteria in Elaeagnus angustifolia from the Western Edge of the Tarim Basin |
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| DOI：doi:10.3969/j.issn.1005-7021.2026.03.005 |
| 中文关键词: 沙枣（Elaeagnus angustifolia） 根际促生菌(PGPR) 促生作用 水解酶活性 叶绿素含量 叶片含氮量 抗氧化酶活性 |
| 英文关键词: Elaeagnus angustifolia plant growth-promoting rhizobacteria(PGPR) growth-promoting effect hydrolytic enzyme activity chlorophyll content leaf nitrogen content antioxidant enzyme activity |
| 基金项目:新疆维吾尔自治区自然科学基金重点项目(2022D01D46) |
| 作者 | 单位 | | 董宇韬 | 1.新疆农业大学 林学与风景园林学院,新疆 乌鲁木齐 830052
2.新疆林业科学院 造林治沙研究所,新疆 乌鲁木齐 830063 | | 何苗 | 2.新疆林业科学院 造林治沙研究所,新疆 乌鲁木齐 830063 | | 聂羽伶 | 1.新疆农业大学 林学与风景园林学院,新疆 乌鲁木齐 830052
2.新疆林业科学院 造林治沙研究所,新疆 乌鲁木齐 830063 | | 罗青红 | 2.新疆林业科学院 造林治沙研究所,新疆 乌鲁木齐 830063 | | 包慧芳 | 4.新疆农业科学院 微生物应用研究所,新疆 乌鲁木齐 830091 | | 吴梓菲 | 3.新疆农业大学 农学院,新疆 乌鲁木齐 830052
4.新疆农业科学院 微生物应用研究所,新疆 乌鲁木齐 830091 | | 王兴雯 | 3.新疆农业大学 农学院,新疆 乌鲁木齐 830052
4.新疆农业科学院 微生物应用研究所,新疆 乌鲁木齐 830091 | | 黄选胜 | 4.新疆农业科学院 微生物应用研究所,新疆 乌鲁木齐 830091 |
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| 中文摘要: |
| 利用植物根际促生菌(plant growth-promoting rhizobacteria，PGPR)促进植物生长发育是一种绿色可持续农业发展的新型策略。为挖掘具有开发潜力的PGPR资源并验证其促生效果，从新疆塔里木西缘岳普湖县沙枣（Elaeagnus angustifolia）根际土壤样品中进行微生物菌种分离，并测定其解磷、固氮、产氨等促生能力，筛选并鉴定促生菌株，测定菌株水解酶活性及生长特性。通过回接试验，测定植株生长生理指标，验证其促生效果。结果显示，从沙枣根际土壤中分离得到66株细菌，其中10 株具备三种及以上促生特性，泛菌属（Pantoea）菌株SZ1具备6种促生特性及4种水解酶活性。回接菌株SZ1至沙枣种子和幼苗后，其发芽势、发芽率、根长、株高、叶片数、茎粗和鲜重较清水对照(CK1)分别提高了76.47%、64.00%、53.61%、50.98%、32.47%、51.49%和51.27%；较空白NB培养基溶液对照(CK2)分别提高了70.45%、65.32%、49.21%、48.98%、30.52%、42.99%和51.94%。此外，叶绿素含量和叶片含氮量较CK1分别提高了26.35%和40.54%，较CK2分别提高了23.84%和35.58%；叶片抗氧化酶活性(SOD、CAT和POD)较CK1分别提升了21.46%、29.18%和33.74%，较CK2分别提升了19.90%、17.82%和31.81%。本研究筛选获得10株具有多种促生功能的根际细菌，其中泛菌属菌株SZ1促生效果显著，为促进植物生长所需的微生物菌剂和微生物肥料的开发提供重要的菌种资源。 |
| 英文摘要: |
| The use of plant growth-promoting rhizobacteria (PGPR) to enhance plant growth and development is a novel, green, and sustainable agricultural strategy. In this study, microbial strains were isolated from rhizosphere soil samples of Elaeagnus angustifolia from the western edge of the Tarim Basin, and evaluated their phosphate-solubilizing, nitrogen-fixing, ammonia-producing, and other growth-promoting abilities. Through the re-inoculation experiment, the growth and physiological indexes of plants were determined to verify their growth-promoting effects. The results showed that 66 bacterial strains were isolated from the rhizosphere soil of Elaeagnus angustifolia, among which 10 strains exhibited three or more plant growth-promoting traits. The Pantoea strain SZ1 possessed six growth-promoting characteristics and showed the activity of four types of hydrolases. After re-inoculation of strain SZ1 into the seeds and seedlings of Elaeagnus angustifolia, the germination potential, germination rate, root length, seedling height, number of leaves, stem diameter, and fresh weight increased by 76.47%, 64.00%, 53.61%, 50.98%, 32.47%, 51.49%, and 51.27%, respectively, compared with the sterile water control (CK1), and by 70.45%, 65.32%, 49.21%, 48.98%, 30.52%, 42.99%, and 51.94%, respectively, compared with the blank NB medium control (CK2). In addition, chlorophyll and leaf nitrogen contents increased by 26.35% and 40.54% over CK1, and by 23.84% and 35.58% over CK2. The activities of antioxidant enzymes (SOD, CAT and POD) in the leaves were also enhanced by 21.46%, 29.18%, and 33.74% compared with CK1, and by 19.90%, 17.82%, and 31.81% compared with CK2. In this study, 10 strains of rhizosphere bacteria with multiple growth-promoting functions were screened, among which the Pantoea strain SZ1 had a significant growth-promoting effect, providing important strain resources for the development of microbial agents/fertilizers required for promoting plant growth. |
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