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| mpc1基因缺失对酵母肌动蛋白等动力学的影响 |
| Effect of mpc1 Gene Deletion on Actin and Other Proteins Dynamics of Schizosaccharomyces pombe |
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| DOI：doi:10.3969/j.issn.1005-7021.2026.02.005 |
| 中文关键词: mpc1 基因 粟酒裂殖酵母 有丝分裂 细胞动力学 肌动蛋白 基因回补 |
| 英文关键词: mpc1 gene Schizosaccharomyces pombe mitosis cell dynamics actin gene complementation |
| 基金项目:四川省科技厅应用基础面上项目(2022NSFSC0107); 四川省科技厅农业科技成果转化资金项目(2022NZZJ0003);四川省转移支付科技成果转移转化示范项目(22ZYZFSF0009，23ZHSF0082) |
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| 摘要点击次数: 34 |
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| 中文摘要: |
| 细胞骨架调控线粒体的融合和分裂，同时线粒体也调控细胞骨架的正常动力学。mpc1基因编码线粒体丙酮酸载体1（mitochondrial pyruvate carrier 1），该蛋白位于线粒体内膜中，是线粒体丙酮酸载体复合物的重要成分。目前，尚未见相关文献报道mpc1基因缺失对酵母肌动蛋白等动力学的影响。本研究对粟酒裂殖酵母（Schizosaccharomyces pombe）的微管、组蛋白、肌动蛋白构建荧光标记，利用活细胞成像技术探究mpc1基因缺失对肌动蛋白等动力学的影响。结果表明，常温下mpc1菌株与野生型菌株相比，纺锤体伸长时间缩短6.70 min（P<0.01）、伸长速率加快0.17 μm/min（P<0.01）；染色体分离时间缩短3.40 min（P<0.01）；肌动蛋白环聚集和收缩总时间延长3.80 min（P<0.01）。mpc1基因缺失虽然促进了纺锤体伸长和染色体分离，但减缓了肌动蛋白环形成和收缩，最终导致细胞生长缓慢。本研究为进一步探究mpc1功能提供参考。 |
| 英文摘要: |
| The cytoskeleton could regulate the fusion and division of mitochondria, meanwhile, mitochondria also could regulate the normal dynamics of the cytoskeleton. The mpc1 gene encodes mitochondrial pyruvate carrier 1 which is located in the mitochondrial membrane. The protein of MPC1 is an essential component of the mitochondrial pyruvate carrier complexes. Currently, the effect of mpc1 gene deletion on the dynamics of spindle, histone, and actin has not been reported. In this study, fluorescent labels of microtubules, histone, and actin were constructed in Schizosaccharomyces pombecells. The stability of cell dynamics at different stages was observed using live-cell imaging to investigate the effects of mpc1 gene deletion on actin. Compared with the wild-type strain, the results showed that the mpc1Δ strain reduced the spindle elongation time by 6.70 min (P<0.01), accelerated the elongation rate by 0.17 μm/min (P<0.01), reduced the chromosome segregation time by 3.40 min (P<0.01), and prolonged the total time for actin ring aggregation and contraction by 3.80 min (P<0.01) at room temperature. The experimental results mentioned above indicated that although the deletion of mpc1 gene promoted spindle elongation and chromosome segregation, it slowed down the formation and contraction of actin rings, ultimately leading to slow cell growth. This study provided a basis for further investigation of mpc1 function. |
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