| In this study a wild fungus strain collected from Chaoyang City, Liaoning Province was identified as Auricularia cornea by morphology and ITS sequence analysis, and its biological characteristics and cultivation and domestication were studied. Under the condition of solid culture, the effects of different carbon sources, nitrogen sources, pH and temperature on the growth of Auricularia cornea mycelium were analyzed. In the cultivation and domestication experiment, the length, width, thickness, fresh weight of single fructification, average yield of three tides per stick of fresh ear were measured, and the biological conversion rate was calculated. The results of single factor test showed that the optimum carbon source for the growth of Auricularia cornea mycelium was soluble starch, the nitrogen source was yeast extract powder and soybean meal powder, pH was 7.0-9.0, and the temperature was 30 ℃. The results of cultivation and domestication test showed that the mycelial growth rate of the control group (78% sawdust,mass fraction,the same below) was about 4 mm/d, the fresh weight of a single fructification was (18.13±6.54) g, the average yield of three tides was (501.91±14.40) g, and the biological conversion rate was (100.38±2.88)%. The mycelial growth rate of the experimental group (50% Lentinus edodes bran, 28% sawdust) was about 4.23 mm/d, the fresh weight of single fruiting body was (11.18±3.17) g, the average yield of three tides was (400.79±19.41) g, and the biological conversion rate was (80.16± 3.88)%. In summary, Lentinus edodes bran can promote mycelial growth, and can successfully cultivate and domesticate wild Auricularia cornea, and ultimately achieve its resource utilization. At the same time, this study also provides a reference for the development and utilization of wild germplasm resources and the breeding of new varieties in China. |
