|
| 回顾Rep与Cap的结构及其对研究 CRESS DNA 病毒的启发 |
| Reviewing the Structures of Rep and Cap Proteins and Their Implications for the Study of CRESS DNA Viruses |
| |
| DOI：doi:10.3969/j.issn.1005-7021.2025.02.014 |
| 中文关键词: CRESS DNA病毒 衣壳蛋白 复制酶相关蛋白 蛋白质结构 致病机制 |
| 英文关键词: CRESS DNA viruses Cap Rep protein structure pathogenic mechanism |
| 基金项目:国家自然科学基金面上项目（31970152）；云浮市科技计划项目（2022020202） |
|
| 摘要点击次数: 28 |
| 全文下载次数: 6 |
| 中文摘要: |
| 编码复制相关蛋白（Replication-associated protein，Rep）的单链环状DNA 病毒（Circular Rep Encoding Single-Stranded DNA （CRESS DNA） Viruses）感染真核生物，具有病毒基因组小、宿主范围多样化、流行率高、滚环复制以及亲和性强等特点。CRESS DNA病毒广泛感染且潜伏于多种真核生物，包括植物、动物、人类和真菌等，在病毒学领域影响深远且具有重要意义。CRESS DNA病毒是一种小型非囊膜DNA病毒，直径15~25 nm。病毒具有单链环状的基因组，主要编码两种蛋白质包括衣壳相关蛋白（Capsid protein, Cap）以及复制相关蛋白。Cap是组成病毒衣壳的唯一蛋白质组分，且在整个病毒复制周期中起着至关重要的作用，其参与病毒附着、细胞进入、基因组脱壳和成熟病毒粒子的组装。Rep则介导病毒基因组复制起始位点的识别，并利用其核酸内切酶活性使环状DNA断裂以进行滚环复制（Rolling cycle replication, RCR）。CRESS DNA 病毒能够利用碱基长度有限的基因组在多种真核生物中进行复制并引起许多重要疾病，是病毒自然进化过程中产生的一种优秀的研究模型，该病毒进行复制的分子机制以及感染宿主的免疫机制值得深入探究。本文回顾了目前对CRESS DNA病毒编码蛋白的结构研究，通过了解蛋白在复制周期中的作用及其相互关系，为进一步阐明CRESS DNA病毒相关疾病的发病机制提供参考。 |
| 英文摘要: |
| The single-stranded circular DNA viruses encoding replication-associated proteins (CRESS DNA viruses) infect eukaryotes and are characterized by a small viral genome, diverse host range, high prevalence, rolling circle replication and strong affinity. CRESS DNA viruses are widely found and latent in various eukaryotes, including plants, animals, humans and fungi, and they have profound implications and significance in the field of virology. CRESS DNA viruses are small non-enveloped DNA viruses with a diameter of 15-25 nm. They possess a single-stranded circular genome that primarily encodes two types of proteins: the capsid protein (Cap) and the replication-associated protein (Rep). Cap is the sole protein component of the viral capsid and plays a crucial role throughout the viral replication cycle, participating in virus attachment, cell entry, genome uncoating and the assembly of mature viral particles. Rep mediates the recognition of the viral genome replication initiation site and uses its endonuclease activity to cleave circular DNA for rolling circle replication (RCR). CRESS DNA viruses can replicate in various eukaryotes using a genome of limited base length and can cause many important diseases. They serve as an excellent model for studying the natural evolution of viruses. The molecular mechanisms of replication and the host immune responses during infection of this virus are worthy of in-depth exploration. This article reviews the current structural studies on CRESS DNA virus encoded proteins and aims to elucidate the roles of these proteins during the replication cycle and their interrelationships, thereby providing a theoretical basis for further clarifying the pathogenesis of CRESS DNA virus-associated diseases. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
