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| 莱州湾沉积物可培养硫氧化细菌多样性研究 |
| Diversity of Cultivable Sulfur-Oxidizing Bacteria in Ocean Sediment of Laizhou Bay |
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| DOI：doi:10.3969/j.issn.1005-7021.2025.02.005 |
| 中文关键词: 莱州湾 沉积物 硫氧化菌 可培养 多样性 弧菌属 高效菌株筛选 |
| 英文关键词: Laizhou Bay sediment sulfur-oxidizing bacteria cultivable diversity Vibrio highly efficient bacteria screening |
| 基金项目:山东省自然科学基金项目（ZR202102280248，ZR2020MC043）；辽宁省教育厅科学研究经费项目（LJ2020QNL021） |
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| 摘要点击次数: 23 |
| 全文下载次数: 12 |
| 中文摘要: |
| 从莱州湾沉积物中分离硫氧化细菌，分析可培养硫氧化细菌多样性并筛选高效硫氧化菌。从莱州湾5个站位的沉积物样品中选择性分离硫氧化细菌，基于16S rRNA基因序列构建系统发育树确定其系统发育地位，并筛选高效硫氧化菌。本研究发现莱州湾沉积物中分布着丰富的硫氧化细菌，其丰度约103 cfu/g，分离鉴定了45株硫氧化细菌，基于16S rRNA基因系统发育关系将其分类为假单胞菌门的γ-变形菌纲、放线菌门、芽胞杆菌门的9个属，其中γ-变形菌纲细菌为优势菌（占比93.62%），优势属（>10%）为Vibrio（弧菌属）、Pseudoalteromonas（假交替单胞菌属）、Pseudomonas（假单胞菌属）。Vibrio是本研究的绝对优势类群（占比51.11%），分布于所有采样点，且最为广泛。以硫代硫酸钠为底物，利用碘量法测定了代表菌株的硫氧化能力，其中12株菌有较高的硫氧化能力（氧化率>70%），其中Vibrio sp. LZB4-9、盐单胞菌属Halomonas sp. LZB2-4、Halomonas sp. LZB4-4的氧化效率最高，在接种后第12天氧化硫代硫酸钠效率达95%以上。本研究揭示了莱州湾沉积物中可培养硫氧化菌多样性，筛选了高效菌株，为修复莱州湾近海养殖区水体及沉积物提供了土著高效菌株，为研究莱州湾硫循环提供了参考。 |
| 英文摘要: |
| This study aims to isolate sulfu-oxidizing bacteria (SOB) from the ocean sediment of Laizhou Bay, evaluate the diversity of cultivable SOB, and screen for highly efficient strains of SOB. The SOB strains were isolated from sediments collected at five sampling sites in Laizhou Bay using sodium thiosulfate as the sole carbon source. All the isolates obtained in this study were classified through phylogenetic analyses based on 16S rRNA gene sequences. The highly efficient strains were screened through iodometric method for each SOB strain. The results indicated abundant SOBs distributed in the sediment of Laizhou Bay, with a richness of approximately 103 cfu/g wet sediment. A total of 45 SOB strains were obtained and were classified into γ-Proteobacteria, Actinomycetota, and Bacillota, with γ-Proteobacteria being the dominant group (accounting for 93.33%). The predominant genera (>10%) were Vibrio, Pseudoalteromonas and Pseudomonas. Vibrio spp. was the most prevalent group (accounting for 51.11%) with the widest distribution and was found at each sampling site. The sulfur-oxidizing capabilities of representative strain revealing that 12 strains exhibited high sulfur-oxidizing efficiency (>70%). Notably, Vibrio sp. LZB4-9, Halomonas sp. LZB2-4 and Halomonas sp. LZB4-4 demonstrated the highest efficiency, oxidizing of sodium thiosulfate reaching above 95% after 12 days of incubation. This study elucidates the diversity of cultivable SOB distributed in Laizhou Bay sediment, contributing to sulfur cycle research and providing native efficient strains for the remediation of sulfur-containing aquaculture water and sediment environments. |
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