| In order to explore the direction of glutamate waste biotransformation slurry resource utilization, a novel technique for utilizing yeast to ferment the slurry with two steps was developed. First, a strain of yeast with high biomass was screened by a medium with addition of the slurry; and the main influencing factors and optimal fermentation conditions was determined by single factor test and response surface optimization experiment. Second, the growth-promoting effect of the fermentation broth on rice, wheat, and maize was evaluated by seed soaking experiment. The results demonstrated that Pichia kudriavzevii X-1 has the highest biomass. The carbon source for Pichia kudriavzevii X-1 fermentation is glucose, and the main effect factors on the fermentation of X-1 were glucose addition, loading volume and incubation time. And the optimal conditions for the fermentation of strain X-1 in the slurry medium were glucose concentration of 90 g/L, shake flask volume of 187 mL/250 mL, and incubation time of 98 hours. The seed soaking experiment results showed that the fermentation broth improved germination of rice, maize and wheat seeds and promoted root growth. Overall, our study provided a valuable direction for development of the slurry in producing yeast strain of X-1 and promoting the growth of graminaceous crops; and provided the theoretical basis and technical support for the bioconversion of glutamate waste slurry at a later stage. |
