| In order to clarify the role of laccase genes in the growth and development of Ganoderma lucidum, in this study, 15 laccase genes of G. lucidum were cloned and bioinformatically analyzed, and the expression levels of the 15 laccase genes were detected at different developmental stages by qRT-PCR. The results showed that the nucleotide sequences of these laccase genes ranged from 1 814-2 570 base pairs in length, contained 4-12 introns, and encoded 517-628 amino acids. Except for Glac14, all laccase genes contained signal peptides. The secondary structures of the 15 laccase proteins mainly were irregular coil and extended strand. All laccase proteins contained conservative domains Cu-oxidase, Cu-oxidase_2, and Cu-oxidase_3. Gllac1, Gllac8 and Gllac10 were highly expressed in the mycelium stage, indicating that they play important roles in the nutrient growth stage. Gllac2, Gllac4, Gllac5, Gllac6, Gllac7, Gllac9, Gllac12 and Gllac13 were highly expressed at the stage of mature fructification, suggesting that they may be related to fructification differentiation and maturation. Gllac3, Gllac11, Gllac14 and Gllac15 were expressed throughout the developmental stages, but the differences were not significant, suggesting that they may play roles throughout the developmental stages of G. lucidum. This study lays a foundation for further elucidating the role of laccase genes in the developmental stages of G. lucidum and developing the application of laccase. |
