| Culicoides arakawae is a common blood-sucking insect in chicken farms which mainly spreads a variety of poultry diseases, such as leucocytozoonosis and avian pox. To understand the culturable midgut bacteria species and community composition of culturable midgut bacteria of C. arakawae, to provide foundation for early stage work research on the influence of midgut bacteria of C. arakawae on disease transmission. C. arakawae collected in Jiangcheng County were used as material to isolate and identify the culturable midgut bacteria. Culicoides were collected using light traps, and non-blood-fed female specimens of C. arakawae were selected. The midgut was dissected and ground, and the culturable bacteria were isolated from the midgut, and identified and analyzed by 16S rDNA sequencing. The results showed that a total of 8 419 culturable midgut bacteria were obtained from the midgut of 4 C. arakawae, and 38 strains were purified, belonging to 10 species of 6 genera, 5 families, and 2 phyla. The dominant bacteria were the gram-positive bacterium, accounting for 57.89% of the total isolated strains, and the dominant strains were Staphylococcus, accounting for 55.26% of the total isolated strains. Culturable midgut bacteria of C. arakawae in Jiangcheng county belong to Proteobacteria and Firmicutes, with Staphylococcus as the main species. |
