| Akkermansia muciniphila strain from a healthy human colostrum was isolated and identified. The strain was enriched in colostrum samples using the medium with mucin, and then isolated by dillution, determining whether the isolated strain was A. muciniphila by morphological observation, specific gene detection, physiological and biochemical detection, as well as 16S rRNA sequence determination and whole genome sequence analysis. The results of bacterial morphology, specificity assay, physiological and biochemical assays from the isolated strain were similar to those of ATCC BAA-835; the 16S rRNA sequence similarity between the isolated strain and ATCC BAA-835 was 99.43%; the characteristics of the whole genome sequence showed that the size of the whole genome is 2 664 112 bp, the GC content is 55.76%, the sequence coding rate is 90.23%, the number of COG homologous proteins is 1 694, the number of CRISPR was 2, the number of phage-related genes were 5, and the average nucleotide identity of the isolated strain and ATCC BAA-835 is 99.97%. Therefore, the isolated bacteria was of Akkermansia muciniphila. |
