| In order to establish a serological detection method for goose astrovirus (GAstV) antibodies, recombinant ORF2 protein from GAstV was successfully expressed by insect baculovirus expression system, and an indirect ELISA method for detecting GAstV antibody was established with ORF2 protein as coating antigen. With proved by specificity and repeatability tests, the established indirect ELISA only reacted with the positive serum of GAstV, but not with the positive serum of other goose susceptible viruses, and inter-and-intra batches of assay repeatability coefficients of variation were below 10%. Sensitivity analysis showed that the sensitivity of ELISA was four times as high as IFA. The clinical sample test results showed that the accordance rate of the ELISA method was as high as 89.8% of the IFA accordance rate. All the above results suggested that the ORF2 protein of GAstV was successfully expressed in baculovirus. It is confirmed that the indirect ELISA method for detecting serum antibody of GAstV established in this study has fine specificity, sensitivity and repeatability, and provided an accurate, rapid and economic testing method for clinical infection detection of GAstV, and can be developed as a detection kit for clinical application and provided a technical to rely on for the development of clinically applicable detection kits. |
