| Human NOLC1 (nucleolar and coiledbody phosphoprotein 1) play an imperative regulation role in the onset and progression of cancer. A recombinant NOLC1 adenovirus vector using the Gateway system was established in order to study the impact of NOLC1 on lung cancer cells. After successfully packaging the NOLC1 adenovirus, and respectively infected normal human embryonic lung cells (HEL) and non-small cell lung cancer cells (A549 cells), to overexpress the NOLC1. The overexpression of NOLC1 had more significant impact on reduction activity, apoptosis increment, and decrement of mitochondrial membrane potential in A549 cells.. Adoption of MTT assay, AnnexinV-APC/PI double-staining assay, and mitochondrial membrane potential assay; it was proved as compared with HEL cells the expression of Caspase family, TNF and receptor family, and BCL2 family genes were detected by Real-time PCR. Real-time PCR was used to measure the expression of genes from the Caspase family, TNF and receptor family, and BCL2 family. It was discovered that, in A549 cells, overexpression of NOLC1 significantly increased the expression of pro-apoptotic genes and decreased the expression of anti-apoptotic genes, among them two key pro-apoptotic proteins, CASP8 and BAX, being significantly up-regulated. However, this effect was not evident in HEL cells. The study results suggested that the overexpression of NOLC1 protein has significant anti-tumor activity against non-small cell carcinoma through a combined effect on the mitochondrial pathway and death receptor pathway. |
