文章摘要
天津市特色农产品沙窝萝卜根际细菌多样性及促生菌研究
Rhizosphere Bacteria Diversity and Growth Promoting Strains of Tianjin City Distinctive Produce Shawo Radish
  
DOI:doi:10.3969/j.issn.1005-7021.2024.02.004
中文关键词: 沙窝萝卜  根际细菌  KEGG功能注释  促生
英文关键词: Shawo radish  rhizosphere bacteria  KEGG functional annotation  growth promotion
基金项目:天津市技术创新引导专项(基金)企业科技特派员项目(20YDTPJC00810);天津市食品质量与健康重点实验室开放基金项目(TJS202105)
作者单位
谭红晓 天津科技大学 食品科学与工程学院天津 300457 
崔康佳 天津科技大学 食品科学与工程学院天津 300457 
王娜娜 天津科技大学 食品科学与工程学院天津 300457 
孙久云 天津科技大学 食品科学与工程学院天津 300457 
路来风 天津科技大学 食品科学与工程学院天津 300457 
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中文摘要:
      为发挥微生物资源在地理标志特色农产品品牌维护中的作用,采用高通量测序技术分析天津市西青区沙窝萝卜根际细菌多样性和群落结构,通过纯培养根际土壤细菌对樱桃萝卜果实质量与黄酮、多酚、维生素C、可溶性糖含量的影响评估根际微生物与沙窝萝卜品质的关系。结果表明沙窝萝卜根际土壤样本中的细菌主要来自5门9属,其中假单胞菌属(Pseudomonas)、芽胞杆菌属(Bacillus)、糖单胞分泌菌属(Saccharimonadales)为核心细菌群落。通过纯培养共获得39株可培养细菌菌株。经过筛选,获得7株具有固氮、解磷或分泌生长素综合水平较高且具有促生功能的菌株,包括菌株J3、J5、H11、X3、X6、X10、X13。根据16S rRNA 基因序列鉴定这些菌株为奇异变形杆菌(Proteus mirabilis)、嗜虫假单胞菌(Pseudomonas entomophila)、巨大芽胞杆菌(Bacillus megaterium)、蜡状芽胞杆菌(B. cereus)、惠州芽胞杆菌(B. huizhouensis)、地形变形杆菌(P. terrae)和粘质沙雷氏菌(Serratia marcescens)。盆栽试验结果表明,施用7株菌株可以促进樱桃萝卜生长或改善果实品质。其中,菌株H11、J5、X13可分别提高樱桃萝卜果实重量47%、45%和42%;菌株H11和J5可提高樱桃萝卜黄酮含量47%和40%,菌株J5和X6可提高樱桃萝卜多酚含量70%和67%;菌株X6对果实中维生素C含量影响较大,较对照组提高了近3倍,达(24.60±5.07) mg/100 g,菌株H11处理后果实中可溶性糖含量较对照组提高了2.7倍。研究结果表明,挖掘沙窝萝卜根际土壤微生物资源有助于利用细菌群落制定有效和可持续的作物田间管理策略。
英文摘要:
      In order to better exertion of microbial resources′ role to maintain the brand of produce with geographical indications, high-throughput sequencing technology was employed to evaluate the structure and composition of the rhizosphere bacterial community of Shawo radish in Xiqing District, Tianjin city. The relationship between rhizosphere microorganisms and the quality of Shawo radish was evaluated by the effect of pure culture of rhizosphere soil bacteria on the quality of cherry radish fruit and the content of flavonoids, polyphenols, vitamin C, soluble sugar. The results showed that Shawo radish possesses rhizosphere bacteria from 5 phyla and 9 genera, of which Pseudomonas, Bacillus and Saccharimonspora were the core bacterial communities. A total of 39 culturable bacterial strains were obtained through pure culture. Seven strains with high nitrogen fixation ability, phosphorus degradation capacity or secretion of auxin and growth-promoting capacity were screened and obtained from the 39 strains, namely strains J3, J5, H11, X3, X6, X10 and X13. Based on 16S rRNA gene sequence, these strains were identified as Proteus mirabilis, Pseudomonas entomophila, Bacillus megaterium, B. cereus, B. huizhouensis, P. terrae, and Serratia marcescens. The results of the pot experiment showed that the application of seven strains could significantly promote the growth of cherry radish plants or improve their quality. Strains H11, J5 and X13 increased the weight of cherry radish fruit by 47%, 45% and 42%. Strains H11 and J5 increased fruit flavonoids by 47% and 40%, while strains J5 and X6 increased polyphenols by 70% and 67%. Strain X6 had advantages in improving vitamin C content (24.60±5.07) mg/100 g in the fruit, and was 3 times higher than control groups. In addition, soluble sugar content in fruit treated with strain H11 was 2.7 times higher than in the control group. The above results indicated that excavating rhizosphere microbial resources around Shawo radish conducive to the utilization of bacterial community to enact effective and sustainable strategies for crop field management.
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