In order to study the microbial diversity in rhizospheric soil of different medicinal plants, the rhizospheric soil of Atractylodes macrocephala, Paeonia sterniana, Paeonia suffruticosa, Scrophularia ningpoensis as well as soil of non-planting area were collected respectively. The V3-V4 region of 16S rRNA gene was targeted and sequenced to analyze the composition of soil microbial community. The results showed that the bacteria diversity index in rhizosphere of medicinal plants was significantly higher than that in non-planting soil. The dominant groups of five groups of samples are not significantly different, and the overall relative abundances of Proteobacteria, Anhydrideobacteria, Actinobacteria, Gemmatimonadetes, and Chloroflexi are relatively high. The relative abundance of Actinomycetes in the rhizosphere of medicinal plants was obviously higher than that in non-planted area. At the genus level, there are greater differences in the community structure between four medicinal plants and the non-planted groups. The rhizosphere soils of the four Chinese herbal medicines were enriched with specific genera of beneficial bacteria. The relative abundance of NMD1, Dongia, Gaiella, and Streptomyces in rhizosphere soil of medicinal plants was obviously higher than that in the non-planting area, and the relative abundance of Lysobacter in soil of the non-planted area was significantly higher than that of medicinal plants. In addition, the functions of nitrate reduction and aromatic compound degradation were enriched in the rhizosphere soil of the four Chinese medicinal materials. |