| As the first successfully cultivated fungus belonging to Boletales, casing soil (CS) is usually used to improve the cultivation. Microorganisms play important roles in the development of fruiting bodies (FB). Present researches mainly focus on microorganisms in casing soil, but lack of exploring endophytic bacteria in fruiting bodies. In order to pierce the bacterial components in the FB of Phlebopus portentosus (Berk. & Broome) Boedijn, 16S rRNA based on Illumina MiSeq platrform high throughput sequencing targeting V3-V4 regions was used to analyze the bacterial population structure in P. portentosus. The results indicated that there were high abundance of bacterial species inside the fruitification of P. portentosus, mainly predominated by Proteobacteria. Cupriavidus, Acinetobacter, Ochrobactrum, Pelomonas, Delftia and Aquabacterium were the most abundant genus. A total of 468 unique OTUs were detected in FB, which occupied 26.65% of the total sequences. Their classification belonged to the genus of Cupriavidus, Acinetobacter and Delftia. In addition, both results of FB and CS difference analysis and Lefse iconic microbial analysis all showed that the genera of Cupriavidus, Acinetobacter, Ochrobactrum, Pelomonas, Delftia and Aquabacterium were the specificity and iconic groups. The results of using Picrust (Phylogenetic investigation of communities by reconstruction of unobserved states) to carried out KEGG (Kyoto Encyclopedia of Genes and Genomes) access prediction found that the main metabolic pathway in FB endophytic bacteria was membrane transmission, amino acid metabolism, carbohydrate metabolism, xenobiotics biodegradation and metabolism, and there were 20 differences of metabolic accesses, moreover, xenobiotics biodegradation and metabolism, signal conduction, other amino acid metabolism, lipid metabolism, cellular process and signal the proportion of these 5 accesses were higher than in CS. The difference of microbial groups may be the reason of these performances differences. |
