| In order to solve the problems of low efficiency of nitrogen removal and poor environmental safety in the traditional constructed wetlands for treating polluted water. The strain TY was isolated and purified from sludge in biochemical treatment plant of sewage treatment plant (CGMCC1.18865. The strain was put into storage in China General Microbiological Culture Collection Center in 2020), through the water distribution test, the denitrification characteristics of the strain were verified, and the volcanic rock was used as the wetland filler, and the wetland simulation system with volcanic rock as filler was used to compare the denitrification effect between the wetland system with TY bacteria and the traditional wetland system; TY strain was identified by 16S rRNA gene sequence analysis, and its denitrification was analyzed and studied. The results showed that the isolated TY bacteria were Pseudomonas sp., and the removal rates of ammonia nitrogen and total nitrogen in water distribution test were 84.2% and 93.6%. Through the monitoring of ammonia nitrogen, total nitrogen and chemical oxygen demand (COD) in the effluent of the simulated wetland system, it is found that the average removal rate in 30 days can increase to 94.3%, 88.0% and 82.3%, which is 27.9%, 28.8% and 7.3% higher than those of the traditional wetland. The research showed that there was a small amount of nitrate and nitrite accumulation in the denitrification process of TY bacteria, which can reduce the adverse impact on the environment and facilitate the continuous denitrification reaction, so as to improve the denitrification efficiency of constructed wetland, which has the prospect of further exploration. |
