文章摘要
微生物暗物质分离培养——以CPR和DPANN类群为例
Isolation and Cultivation of Microbial Dark Matter—Take CPR and DPANN Group as Examples
  
DOI:doi:10.3969/j.issn.1005-7021.2023.02.011
中文关键词: 微生物暗物质  候选门级辐射类群  DPANN  细胞微小  共生生活  分离培养
英文关键词: microbial dark matter  candidate phyla radiation  DPANN  small cell size  symbiotic lifestyles  isolation and cultivation
基金项目:内蒙古自然科学基金项目(2020BS03006);内蒙古师范大学引进高层次人才科研启动经费项目(2018YJRC015)
作者单位
姜凯 1.内蒙古师范大学 生命科学与技术学院内蒙古 呼和浩特 0100222.内蒙古自治区高等学校生物多样性保护与可持续利用重点实验室内蒙古 呼和浩特 010022 
曹春玲 内蒙古自治区农牧业技术推广中心内蒙古 呼和浩特 010010 
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中文摘要:
      候选门级辐射类群(candidate phyla radiation, CPR)细菌和DPANN超门古菌是重要的微生物暗物质,约占地球细菌和古菌多样性的一半。目前对于CPR和DPANN的研究处于起步阶段,仅获得了少数的实验室培养菌株,代表着丰富的菌种资源和基因资源的潜力;同时其生态功能还未知,预示着未来新发现的巨大机遇。本文在总结现有微生物分离培养方法以及CPR和DPANN菌株共性特征的基础上,对已实现实验室培养的CPR和DPANN菌株进行归纳和分析,提出该类群菌株分离培养的几点建议,以期为今后对该类群菌株的分离培养提供参考。CPR细菌和DPANN古菌的可培养研究有助于加深对该类群菌的认识,阐明其生活方式、进化及演变规律并揭示其独特的代谢途径及功能基因,发现新的天然活性产物,具有重要的科学意义及应用价值。
英文摘要:
      Candidate phyla radiation (CPR) bacteria and DPANN superphyla archaea are important microbial dark matter, accounting for approximately half of all the bacterial and archaeal diversity of the planet. At present, their researches are in the initial stage. Only a few cultured strains in laboratory of CPR and DPANN have been obtained so far. They represent rich resource of strains and genes, while their ecological functions are unknown, representing great opportunities for new discovery in the future. Based on introduction of the existing methods of microbial isolation and culture and common characteristics of CPR and DPANN, the CPR and DPANN strains that have been realized in cultivation in laboratory were summarized and analyzed, and some suggestions for the isolation and cultivation of the two groups were put forward, so as to provide reference for the future isolation and cultivation. The cultivation study on CPR and DPANN is helpful to deepen the understanding of the two groups, clarify their lifestyle and evolution rules, reveal their unique metabolic pathway and functional genes, and discover new natural active products, which has important scientific significance and application value.
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