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| 细菌高通量数据荟萃分析的可行性研究 |
| Feasibility of Meta-Analysis on Bacterial High-Throughput Data |
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| DOI：doi:10.3969/j.issn.1005-7021.2023.02.010 |
| 中文关键词: 高通量测序 荟萃分析 扩增引物 16S rRNA 截取引物 |
| 英文关键词: high-throughput sequencing meta-analysis amplification primers 16S rRNA interceptive primers |
| 基金项目:四川省科技厅应用基础重点项目（2020YJ0346）；西华师范大学博士科研启动项目（19E048）；四川省自然科学基金项目（2023NSFSC1223） |
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| 中文摘要: |
| 细菌作为重要的分解者，对于生态系统功能至关重要，其群落多样性一直是生态研究的热点。通过荟萃分析，整理已发表的细菌高通量数据，已经取得一些重要的全球性研究结果。这些荟萃分析的核心是将不同引物来源序列提取统一区段，进行细菌群落分析，但该方法的可行性和准确性还一直未被探究。本研究使用了含有三种引物扩增的同一细菌16S rRNA高通量数据，分析了不同引物和提取统一区段后细菌群落情况。结果显示，不同引物以及提取统一区段后，得到细菌群落组成和多样性显著不同，且提取同一区段后的群落主要受原始引物的影响较大。该研究表明，细菌高通量数据荟萃分析中，引物差异会影响其统一分析的群落结果，荟萃方法需要慎重使用。 |
| 英文摘要: |
| Bacteria, as important decomposers, are crucial to ecosystem functions, and their community diversity has always been a hotspot in ecological research. The global research results has achieved some important findings about microbial ecology by sorting out published bacterial high-throughput data adopting meta-analysis. The core of these meta-analyses is to implement bacterial community analyses of unified sections extracted from different primer resource sequences, however the feasibility and accuracy of the approached has not been researched. In this study, an amplified identical bacterial 16S rRNA high-throughput data, containing three different primers, to analyze bacterial communities extracted from different primers and uniform sections. The results showed that different primers as well as after the extraction of the uniform sections, the obtained composition of bacterial population and diversity was significantly different, and the population after the extraction of uniform sections was affected mainly and greatly by the original primers. This study suggested that meta-analysis of bacterial high throughput data, the primer difference will affect the population results, and the assemble method needs to be used prudently. |
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