| In order to reveal the composition, diversity and function of fungal community in bottom mud of different vegetation types at Shaanxi Baoji Qianwei Zhihui National Wetland Park, to provide bases for optimal selection of aquatic plants in the park, bottom mud samples of the typical plant species: common reed (Phragmites australis), narrow leaf cattail(Typha angustiolia), cogonggrass (Imperata cylindrica), scirpus validus (Schoenoplectus tabernaemontani) and pygmy waterlily (Nymphaea tetragona) were used as research objects. Fungal ITS1 region was sequenced adopting next-generation high-throughput sequencing, and obtained fungal community structure components in bottom mud to predict their function informations. And determine their physicochemical properties and enzyme activities. The results showed that 11,778 OTUs (Operational Taxonomic Units) were obtained through sequencing, and they were classified into 34 phyla, 58 classes, 134 orders, 244 families and 599 genera. The fungal populations mainly were Ascomycota and Basidiomycota. The fungal diversity in bottom mud of common reed was the lowest. The relative abundance and sucrase and phosphatase activities of Ascomycetes significantly assumed as positive correlation (P<0.05). The relative abundance, total carbon, and total organic carbon content of Basidiomycota significantly assumed as positive correlation (P<0.05). The fungal communities in bottom mud mainly included three types of nutrient and six types of cross-nutrient functional population. This study approached the differences in fungal community structure, diversity, and potential functions in bottom mud of different plant communities in wetlands, analyzed the effect of correlative physicochemical properties to provide an important theoretical bases, so as to screen plants for artificial wetlands and effectively deployed wetland resources and ecological redemption. |
