| Inhibition of verapamil on quorum sensing (QS) against Pseudomonas aeruginosa(PA) was observed. The minimal inhibit concentration (MIC) of verapamil was determined to establish its culture environment, observed the effect of verapamil on PA growth, and drew the growth curve; The expressions of PA elastase, proteolytic enzymes, and pyocyanin were observed respectively during virulence factor experiment. The results showed that In vitro verapamil showed good antibacterial activity against PA when MIC50 of verapamil was within 128 μg/mL and MIC90 512 μg/mL; the MICRange was 128-512 μg/mL, suggested that it had comparative good inhibition activity; and the growth curve suggested that when the verapamil was at 16 μg/mL it started to present inhibition against PA growth, as the increment of the concentration, the inhibition was gradually increased; when the concentration of verapamil was at 512 μg/mL, 256 μg/mL, 128 μg/mL, 64 μg/mL, 32 μg/mL, and 16 μg/mL, the activity of elastase was significantly inhibited (P<0.01), and elastase had certain inhibition at 8 μg/mL (P<0.05). The concentration of verapamil was at 512 μg/mL, 256 μg/mL, 128 μg/mL, 64 μg/mL, and 32 μg/mL significantly inhibited the expression of pyotoxin and proteolytic enzymes (P<0.01), and it also had certain inhibition at 16 μg/mL (P<0.05). Inhibition of verapamil had concentration dependence against PA. Verapamil had significant effect on QS of PA growth in vitro, and can be developed as potential synergist medicine of antibacterial one. |
