文章摘要
多株功能菌种混合培养条件的响应面优化及其生长关系
Optimization of Mixed Cultivation Conditions with Response Surface Methodology and Growth Relationships of Multiple Functional Strains
  
DOI:doi:10.3969/j.issn.1005-7021.2022.03.004
中文关键词: 光合细菌  枯草芽胞杆菌  植物乳酸杆菌  混合培养条件优化  生长关系
英文关键词: photosynthetic bacteria  Bacillus subtilis  Lactobacillus plantarum  optimization of mixed-cultivation conditions  growth relationship
基金项目:山西省重点研发计划项目(201903D321083);中央引导地方科技发展资金项目(YDZX20201400001443);中北大学研究生科技立项(20201767);中北大学大学生创新创业项目(20210381)
作者单位
白红娟 中北大学 环境与安全工程学院 山西 太原 030051 
卫燕红 中北大学 环境与安全工程学院 山西 太原 030051 
李斌 中北大学 环境与安全工程学院 山西 太原 030051 
王海宾 中北大学 环境与安全工程学院 山西 太原 030051 
杨官娥 山西医科大学 药学院 山西 太原 030001 
蒋雪琴 中北大学 环境与安全工程学院 山西 太原 030051 
胡锦俊 中北大学 环境与安全工程学院 山西 太原 030051 
宋雨 中北大学 环境与安全工程学院 山西 太原 030051 
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中文摘要:
      对3种具有土壤中多种重金属污染修复和病虫害防治功能的光合细菌(Photosynthetic bacteria,PSB,包括Rhodobacter sphaeroides H菌株、Rhodopseudomonas palustris N菌株和Rhodospirillium rubrum M菌株)、枯草芽胞杆菌(Bacillus subtilis, BS)和植物乳酸杆菌(Lactobacillus plantarum, LP)进行混合培养,利用单因素和响应面试验优化培养基配方以及培养条件。确定功能菌混合培养的最佳条件:以光合细菌培养基为基础培养基,外加碳源为10 g/L蔗糖、氮源为1.25 g/L硫酸铵、培养温度为31.5 ℃、初始pH值为7.1、接种量为接种后的初始培养活菌数1.90×109cfu/mL、培养时间为28.0 h。通过功能菌的加性模型法、生长动力学以及混合培养的生长关系分析表明,光合细菌、枯草芽胞杆菌和植物乳酸杆菌的生长均存在互惠互利关系。
英文摘要:
      Three kinds of photosynthetic bacteria (PSB for short, including Rhodobacter sphaeroides H strain,Rhodopseudomonas palustris N strain, and Rhodospirillium rubrum M strain), Bacillus subtilis (BS) and Lactobacillus plantarum (LP), which have the functions of repairing multiple heavy metal pollution in the soil and preventing, controlling diseases, and insect pests, were mixed-cultivated. The medium formula and culture conditions were optimized by single-factor and response surface tests. The results showed that the optimal conditions for the mixed-culture of bacteria were as follows: photosynthetic bacteria culture medium as the basic medium with additional 10 g/L sucrose as carbon source, 1.25 g/L ammonium sulfate as nitrogen source, culture at 31.5 ℃, initial pH 7.1, inoculation volume was equal to the initial cultured bacteria content after inoculation 1.90×109 cfu/mL, and culture for 28.0 h. Additive model method, the modified Gompertz equation, and analysis of growth characteristics of mixed-culture of functional bacteria showed all growth relationship of photosynthetic bacteria, Bacillus subtilis, and Lactobacillus plantarum were mutual beneficial.
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