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| 野野村氏菌FIM02-765的分类鉴定及Simaomicin α的抗肿瘤活性 |
| Taxonomy Identification of Nonomuraea sp. FIM02-765 and Antitumor Activity of Simaomicin α |
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| DOI：doi:10.3969/j.issn.1005-7021.2021.04.006 |
| 中文关键词: 野野村氏菌（Nonomuraea sp.） 分类鉴定 代谢产物 稠环氧杂蒽酮 抗肿瘤活性 |
| 英文关键词: Nonomuraea sp. taxonomy identification metabolic products thick epoxy heteroanthrone antitumor activity |
| 基金项目:福建省自然科学基金项目（2016J01175）；福建省中青年教师教育科研项目（JA15715）；泉州市指导性科技计划项目（2015ZD35） |
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| 中文摘要: |
| 明确海洋沉积物来源的放线菌FIM02-765的分类地位，揭示其代谢产物Simaomicin α的抗肿瘤活性。通过形态学、生理生化特征、细胞糖分组成分析以及16S rRNA序列分析，对海洋放线菌FIM02-765进行菌种鉴定；通过大孔吸附树脂柱层析和Sephadex LH-20凝胶柱层析从该菌的发酵产物中分离得到稠环氧杂蒽酮类化合物Simaomicin α；通过MTT法对化合物Simaomicin α的体外抗肿瘤细胞活性进行了研究。结果表明，菌株FIM02-765属于野野村氏菌（Nonomuraea sp.），同时该菌产生的稠环氧杂蒽酮类化合物Simaomicin α具有较强体外抑制多种肿瘤细胞增殖的活性。研究表明1株经鉴定的海洋野野村氏菌FIM02-765产生稠环氧杂蒽酮类化合物Simaomicin α对肿瘤细胞具有较强体外抑制活性，为深入开展该菌的遗传育种以及Simaomicin α的生物合成研究提供参考。 |
| 英文摘要: |
| This study made clarified the taxonomy identification standing of an actinomycete FIM02-765 from marine sediment source and revealed its metabolite of simaomicin α potent antitumor activities. Adopting morphological observation, physiological and biochemical characteristics, cell sugar composition analysis, combined with 16S rRNA gene sequence analysis, the strain FIM02-765 was identified; adopting macrosporous adsorption resin column chromatography, and Sephadex LH-20 gel chromatography, from the fermentation product of the strain obtained a thick epoxy heteroanthrone compound simaomicin α; MTT assay was applied to study the antitumor activities of the compound simaomicin α against tumor cells in vitro. The results showed that cultural characteristics, physiological and biochemical characteristics as well as 16S rRNA gene sequence analysis had confirmed that this strain belonged to the genus Nonomuraea. Furthermore, a polycyclic xanthone-type compound of simaomicin α produced by this actinomycete exhibited in vitro potent anti-proliferative activities against several tumor cells. Therefore, marine-derived strain FIM02-765 was identified and belonged to Nonomuraea sp. had ability to produce a polycyclic xanthone-type compound simaomicin α which had in vitro potent anti-proliferative activities against multiple tumor cells. This study has established an important foundation for deep research of genetic breeding of strain FIM02-765 and biosynthesis of the compound simaomicin α. |
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