| Characteristics and transmission blocking ability of PbPH were studied by preparing monoclonal antibodies (mAb) against PbPH. The expression phase and the specificity of mAb of PbPH were mainly detected by Western blot (WB) and indirect immunofluoscent assay (IFA). In vitro assays, 3 days after Plasmodium berghei infected female BALB/c mice, the blood of the infected mice was obtained from the tail vein, and mixed culture with the anti-PbPH mAb/PBS at different dilution concentrations (15,110,150), and the number of exflagellation centers of male gametocytes and the number of ookinete were observed and counted 15 min after. Anti-PbPH mAb can identify the surface antigens of male and female gametocytes, male gametes, female gametes/zygotes, retort and ookinetes by WB and IFA. in vitroexperiment of transmission blocking, compared with the PBS control group, after adding different concentrations (15,110) of anti-PbPH mAb, the exflagellation centers of male gametocytes were significantly decreased by 41.7% and 32.7% respectively with difference possessing statistic significance (P<0.05). The number of ookinete was significantly reduced by 45.1% and 14.8%, respectively with difference possessing statistic significance (P<0.05). Anti-PbPH mAb can effectively block the exflagellation centers and ookinetes 〖WTBX〗In vitro, thereby affecting further development and continuous transmission of plasmodium in mosquito′s bodies. |
