| Microbial community structure and diversity in salinized soil in Wuerhe area of northern Xinjiang were studied, so as to discover new strains of salt-tolerant microbial resources in high-salt environment. Culturable salt-tolerant strains were obtained adopting traditional isolation and culture methods, morphological characteristics, 16S rRNA gene sequencing, salt tolerance features, high-throughput sequencing technology were simultaneously employed to analyze the diversity and community structure of salt-tolerant bacteria in Wuerhe area in Xinjiang. The results showed that 11 strains isolated belonged to 6 genera and 11 species, all moderate salt-tolerant strains, with the genus of Bacillus as the dominant ones. On the other hand, a total of 290 952 sequences bands of salinized microbial 16S rRNA(V3-V4) gene sequencing were obtained, they respectively belonged to 24 phyla and 410 genera. Among them Proteobacteria (60.31%), Firmicutes (21.52%), Bacteroidetes (6.9%), and Actinobacteria (6%) with relatively high abundance. The dominant genera were Kluyvera(21%), Hafnia-Obesumbacterium(19.6%), and Pseudomonas(7.5%). Therefore, the dominant bacterial community in salinized soil in Wuerhe area in Xinjiang Bacillus is in the majority, with complex community structure, and rich with potential available microbial resources. Therefore, it is of great significance for further study on new resources of salt-tolerant microorganisms in high-salt extreme environment. |
