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| 昭苏县野生羊肚菌根际土壤细菌多样性研究 |
| Bacterial Diversity in Rhizosphere Soil of Wild Morels in Zhaosu County |
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| DOI：doi:10.3969/j.issn.1005-7021.2020.04.004 |
| 中文关键词: 宏基因组 人工驯化 根际土壤微生物 固氮菌 |
| 英文关键词: megagenomics artificial domestication rhizosphere soil microbes nitrogen-fixing bacteria |
| 基金项目:伊犁师范大学微生物重点实验室科研项目(YLUKLM2018001) |
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| 中文摘要: |
| 采集昭苏县野生羊肚菌（Morchella esculenta根际水平直径10 cm、深度 0~30 cm范围内的3层各10 cm 土壤样品，利用Illumina miseq 2×300 bp高通量测序技术和生物信息学分析方法检测细菌类群的多样性。结果显示，细菌群落Chao和Shannon指数在0~30 cm土层中的平均值分别为152和3.68，且两种指数在10~20 cm土层均最高；细菌群落在各分类水平上的类群（最大类群丰度）分别为变形菌门（Proteobacteria，36.3%）、厚壁菌门（Firmicutes）和拟杆菌门（Bacteroidetes）等13门；黄杆菌科（Flavobacteriaceae，8.4%）、芽胞杆菌科(Bacillaceae)和葡萄球菌科(Staphylococcaceae)等43科；葡萄球菌属(Staphylococcus，7.1%）、杆状菌属(Bacillus)和Cloacibacterium等52属；不能培养的α-变形杆菌（uncultured α-proteobacterium，2.0%）、不能培养的嗜甲基菌科1种（uncultured Methylophilaceae bacterium）和路西法芽胞杆菌(Bacillus luciferensis)等44种。检测到固氮菌32属，包括15属自生固氮菌、9属共生固氮菌和8属联合固氮菌。结果表明，羊肚菌土壤根际微生物样品中细菌类群具有多样性，10~20 cm土层根际微生物多样性较高，但很多种类丰度较低；该土样含有丰富的固氮菌，这对栽培羊肚菌如何选地或覆土具有一定的指导意义。 |
| 英文摘要: |
| The diversity of bacterial community of soil samples around the wild morel rhizosphere with horizontal diameter of 10 cm and depth of 0-30 cm, within the range of three layers of each 10 cm collected in Zhaosu County was detected using Illumina miseq 2×300 bp high-throughput sequencing technology and bioinformatics analysis. The results showed that the average value of Chaos and Shannon indices of the bacterial community in the 0-30 cm rhizosphere soil layer was 152 and 3.68, respectively, and both of them in 10-20 cm layer soil were the highest; and the bacterial communities on various levels of communities (the biggest community with the most abundance) respectively were Proteobacteria (36.3%), Firmicutes and Bacteroidetes and other 13 phyla; and Flavobacteriaceae (8.4%), Bacillaceae, and Staphylococcaceae and other 43 families; and Staphylococcus (7.1%), Bacillus and Cloacibacterium and other 52 genera, and unculturable α-Proteobacterium (2.0%), unculturable Methylophilaceae, a kind of Bacillus luciferensis and other 44 species. There were detected 32 genera of nitrogen fixing bacteria, including of 15 genera of self growing, 9 genera of symbiotic nitrogen fixing bacteria, and 8 genera of associative nitrogen-fixing. Therefore, bacterial community in rhizosphere soil of Morchella had diversity, and the microbial diversity, and rhizospheric microbes of 10-20 cm layer soil were higher than those of other soil layers, however, the abundance of many species was relatively low; the soil samples contained abundant nitrogen-fixing bacteria, and these possessed a certain instructive significance how to choose soil and soil dressing for cultivation of morel. |
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