文章摘要
昭苏县野生羊肚菌根际土壤细菌多样性研究
Bacterial Diversity in Rhizosphere Soil of Wild Morels in Zhaosu County
  
DOI:doi:10.3969/j.issn.1005-7021.2020.04.004
中文关键词: 宏基因组  人工驯化  根际土壤微生物  固氮菌
英文关键词: megagenomics  artificial domestication  rhizosphere soil microbes  nitrogen-fixing bacteria
基金项目:伊犁师范大学微生物重点实验室科研项目(YLUKLM2018001)
作者单位
杨晓绒 伊犁师范大学 生物与地理科学学院, 新疆 伊宁 835000 
赖晓辉 伊犁师范大学 生物与地理科学学院, 新疆 伊宁 835000 
吾尔恩·阿合别尔迪 伊犁师范大学 生物与地理科学学院, 新疆 伊宁 835000 
焦子伟 伊犁师范大学 生物与地理科学学院, 新疆 伊宁 835000 
张相锋 伊犁师范大学 生物与地理科学学院, 新疆 伊宁 835000 
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中文摘要:
      采集昭苏县野生羊肚菌(Morchella esculenta根际水平直径10 cm、深度 0~30 cm范围内的3层各10 cm 土壤样品,利用Illumina miseq 2×300 bp高通量测序技术和生物信息学分析方法检测细菌类群的多样性。结果显示,细菌群落Chao和Shannon指数在0~30 cm土层中的平均值分别为152和3.68,且两种指数在10~20 cm土层均最高;细菌群落在各分类水平上的类群(最大类群丰度)分别为变形菌门(Proteobacteria,36.3%)、厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes)等13门;黄杆菌科(Flavobacteriaceae,8.4%)、芽胞杆菌科(Bacillaceae)和葡萄球菌科(Staphylococcaceae)等43科;葡萄球菌属(Staphylococcus,7.1%)、杆状菌属(Bacillus)和Cloacibacterium等52属;不能培养的α-变形杆菌(uncultured α-proteobacterium,2.0%)、不能培养的嗜甲基菌科1种(uncultured Methylophilaceae bacterium)和路西法芽胞杆菌(Bacillus luciferensis)等44种。检测到固氮菌32属,包括15属自生固氮菌、9属共生固氮菌和8属联合固氮菌。结果表明,羊肚菌土壤根际微生物样品中细菌类群具有多样性,10~20 cm土层根际微生物多样性较高,但很多种类丰度较低;该土样含有丰富的固氮菌,这对栽培羊肚菌如何选地或覆土具有一定的指导意义。
英文摘要:
      The diversity of bacterial community of soil samples around the wild morel rhizosphere with horizontal diameter of 10 cm and depth of 0-30 cm, within the range of three layers of each 10 cm collected in Zhaosu County was detected using Illumina miseq 2×300 bp high-throughput sequencing technology and bioinformatics analysis. The results showed that the average value of Chaos and Shannon indices of the bacterial community in the 0-30 cm rhizosphere soil layer was 152 and 3.68, respectively, and both of them in 10-20 cm layer soil were the highest; and the bacterial communities on various levels of communities (the biggest community with the most abundance) respectively were Proteobacteria (36.3%), Firmicutes and Bacteroidetes and other 13 phyla; and Flavobacteriaceae (8.4%), Bacillaceae, and Staphylococcaceae and other 43 families; and Staphylococcus (7.1%), Bacillus and Cloacibacterium and other 52 genera, and unculturable α-Proteobacterium (2.0%), unculturable Methylophilaceae, a kind of Bacillus luciferensis and other 44 species. There were detected 32 genera of nitrogen fixing bacteria, including of 15 genera of self growing, 9 genera of symbiotic nitrogen fixing bacteria, and 8 genera of associative nitrogen-fixing. Therefore, bacterial community in rhizosphere soil of Morchella had diversity, and the microbial diversity, and rhizospheric microbes of 10-20 cm layer soil were higher than those of other soil layers, however, the abundance of many species was relatively low; the soil samples contained abundant nitrogen-fixing bacteria, and these possessed a certain instructive significance how to choose soil and soil dressing for cultivation of morel.
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