| The aim of this study is to obtain a group of microbial community that efficiently degrade cellulose and produce methane; vegetable anaerobic digestion liquid, molasses anaerobic digestion liquid and pond deposited mud as strain sources, and subcultured with filter paper as carbon source at 55 ℃, the content of methane was determined, finally obtain a group of methane producing microbial community (MC). The MC could effectively decompose the filter paper, the relative decomposition rate of the filter paper could reach as high as 67.3%, the cumulative production of methane could reach as high as 46.5% (volume ratio) within 7 days, the highest activity of carboxymethyl cellulase (CMC) was 26.3 U/mL on the third day of culture. The yield of acetic acid was the highest among organic acids, and the cumulative amount in 7 days was 2.7 g/L. High throughput sequencing analysis based on 16S rRNA gene amplification showed that the bacterial diversity was higher than that of archaea. The bacterial community was mainly composed of Lutispora, Aeribacillus, Aneurinibacillus, Symbiobacterium, Clostridium,etc. among them Lutispora was the dominant community, accounting for 11.04% of the total bacterial abundance. The archaeal community mainly included Methanothermobacter, Methanothrix, Methanobacterium, Methanospirillum, etc, among them Methanothermobacter was the dominant archaeal community accounts for 99.82% of the total abundance of archaea. This group of methane-producing bacterial community with high-efficient decomposing cellulose could attain the degradation of cellulose to produce methane through coordinative actions of various microorganisms. |
