文章摘要
峨眉山野生金蝉花孢梗束培养试验
Coremium Culture Experiment of Wild Cicada From Mt. Emei
  
DOI:doi:10.3969/j.issn.1005-7021.2020.03.014
中文关键词: 金蝉花  培养基工艺优化  回归分析  孢梗束的多变性  营养成分
英文关键词: Cordyceps cicadae  medium technology optimization  regression analysis  variation of coremium  nutritional components
基金项目:国家级大学生创新创业训练计划资助项目(201810619039)
作者单位
李燕秋 西南科技大学生命科学与工程学院 生物工程系四川 绵阳 621010 
贺小丽 西南科技大学生命科学与工程学院 生物工程系四川 绵阳 621010 
庄在非 西南科技大学生命科学与工程学院 生物工程系四川 绵阳 621010 
贺新生 西南科技大学生命科学与工程学院 生物工程系四川 绵阳 621010 
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中文摘要:
      为确定峨眉山金蝉花孢梗束培养的最佳工艺条件,对金蝉花孢梗束生长的常用培养基配方进行筛选试验,选用大米、小米、黄豆等原料进行配方试验,采用均匀设计试验法优化培养基配方。结果表明,金蝉花在以无机盐为主要营养成分的Richard琼脂培养基和Czapek琼脂培养基上均能产生孢梗束。在大米等的配方试验中,以大米为营养物质的金蝉花孢梗束生长最快,金蝉花氨基酸含量为23.4%,粗多糖含量为26.5%,蛋白质含量为29.3%,均高于野生金蝉花中该物质的含量。在均匀设计的配方试验中,10号培养基中的金蝉花孢梗束以珊瑚状为主,菌体形态最好,孢梗束产率可达57.4%。10号配方孢梗束中蛋白质的含量高达42.5%,氨基酸含量高达33.3%,均远高于野生金蝉花。
英文摘要:
      In order to determine the optimum technological conditions of coremium of Cordyceps cicadae in Mt. Emei, screening tests were carried out on common medium formulas for the growth of coremium of C. cicadae. Rice, millet, soybean and other raw materials were selected for the formulation tests, and a uniform design test method was used to optimize the medium formulas. The results showed that both Richard agar medium and Czapek agar medium with inorganic salts as the main nutrients could produce coremium. In the experiment formula with rice, the coremium of C. cicadae with rice as nutrient grew fastest, and the content of amino acid, crude polysaccharide and protein in C. cicadae were 23.4%, 26.5% and 29.3% respectively, all were higher than those grew in wild. In the uniformly designed formula experiment, the coralline coremium was the main form in medium No. 10, the best shape of thalamus, with coremium yielding rate could reach up to 57.4%. The content of protein and amino acid in coremium of formula 10 was as high as 42.5% and 33.3%, all were much higher than those of wild C. cicadae.
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