文章摘要
Zn2+对EGCG的结构和抑菌活性的影响
Effects of Zn2+ on Structure and Bacteriostasis Activity of EGCG (Epigallocatechin-3-Gallate)
  
DOI：doi:10.3969/j.issn.1005-7021.2020.03.010
中文关键词: EGCG  EGCG-Zn2+  同分异构体  抑菌作用
英文关键词: EGCG  EGCG-Zn2+  isomer  bacteriostasis
基金项目:辽宁省自然科学基金项目（20180550231）
作者单位
苗嘉桐 大连医科大学基础医学院 生物技术系，辽宁 大连 116044 
侯兴栋 大连医科大学 附属第二医院，辽宁 大连 116044 
夏思远 大连医科大学基础医学院 生物技术系，辽宁 大连 116044 
邓忆美 大连医科大学 附属第二医院，辽宁 大连 116044 
付维哲 大连医科大学基础医学院 生物技术系，辽宁 大连 116044 
孙铁英 大连医科大学基础医学院 生物技术系，辽宁 大连 116044 
姜涛 大连医科大学基础医学院 生物技术系，辽宁 大连 116044 
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中文摘要:
      揭示耻垢分枝杆菌（Mycobacterium smegmatis mc2155）和Zn2+对表没食子儿茶素没食子酸酯（Epigallocatechin-3-gallate，EGCG）结构和生物学活性的影响，为开发高活性EGCG制剂奠定基础。 首先利用HPLC检测EGCG和耻垢分枝杆菌相互作用后的含量和结构变化,进而化学合成EGCG-Zn2+，利用紫外吸收光谱法和HPLC方法鉴定EGCG被Zn2+修饰前后的结构变化。最后采用纸片琼脂扩散法比较EGCG和EGCG-Zn2+对耻垢分枝杆菌的抑制作用。EGCG与耻垢分枝杆菌相互作用18 h后，即出现不同的EGCG代谢物，且EGCG自身结构含量比例明显降低。EGCG与特定金属离子Zn2+结合后，其最大吸收波长和吸收强度均有改变，且EGCG-Zn2+在紫外吸收光区有明显的酚羟基吸收。HPLC结果表明, EGCG-Zn2+能引起EGCG结构变化而导致保留时间延长。抑菌实验研究发现，EGCG-Zn2+对耻垢分枝杆菌的抑菌作用弱于EGCG。研究表明，EGCG结构的稳定性容易受到细菌以及Zn2+的影响，为EGCG的进一步研究开发提供线索。
英文摘要:
      The effect of Mycobacterium smegmatis mc2155 and Zn2+on the structure and biological activities of EGCG (Epigallocatechin-3-gallate) the content and structure of EGCG were firstly detected by HPLC after interacting EGCG and M. semgmatis mc2155. Secondly, EGCG-Zn2+ was chemo-synthesized, its structure was identified its structural changes before and after decorated with Zn2+using ultraviolet absorption spectrometry and HPLC. Finally the bacteriostasis of EGCG was compared with EGCG-Zn2+ adopting paper disc agar diffusion method. The results showed that different kinds of EGCG metabolites appeared immediately after interacted with M. smegmatis mc2155 for 18 h, and the proportion of the structural content of EGCG with itself was decreased obviously. The maximum absorption wavelength of EGCG was altered after combined with specific metal ions Zn2+, and both of its maximum absorption wavelengths and absorption strength had changed, and there was an obvious phenolic hydroxyl absorption in ultraviolet absorption area. The results of HPLC showed that EGCG-Zn2+could cause EGCG structure to change and beget the retention time of EGCG to prolong. Bacteriostatic experiment and study had found that the effect of EGCG-Zn2+ on M. smegmatis mc2155 was weaker as compared to that of EGCG. Therefore, this study indicated that the stability of EGCG structure was subjected to bacteria and Zn2+, and provided clue for further research and the development of EGCG.
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