文章摘要
血吸虫感染通过抑制前炎症免疫应答缓解实验性脑疟的发生
Suppression of Pro-Inflammatory Immune Response by Schistosomiasis Infection to Alleviate Experimental Cerebral Malaria
  
DOI:doi:10.3969/j.issn.1005-7021.2020.03.009
中文关键词: 前炎症免疫应答  混合感染  实验性脑疟  日本血吸虫
英文关键词: pro-inflammatory immune response  combined infection  experimental cerebral malaria  Schistosoma japonicum
基金项目:国家自然科学基金项目(81401285)
作者单位
冯永辉 中国医科大学附属第一医院 检验科辽宁 沈阳 110001 
曹雅明 中国医科大学基础医学院 免疫学教研室辽宁 沈阳 110122 
朱晓彤 中国医科大学基础医学院 免疫学教研室辽宁 沈阳 110122 
摘要点击次数: 355
全文下载次数: 104
中文摘要:
      脑疟所引起的严重神经系统的并发症是引起5岁以下儿童死亡的主要原因。疟疾宿主体内免疫应答紊乱是引起脑疟发生的重要机制。目前在疟疾流行区,疟疾患者常常伴有蠕虫感染,但感染虫株类型,严重程度以及感染途径不同均会影响感染结局。为探讨前炎症免疫应答在混合感染中的作用地位,采用日本血吸虫和ECM模型来探讨相关免疫机制。通过建立混合感染模型,监测原虫血症水平和宿主生存期;FACS检测脾脏中DC亚群、巨噬细胞、Treg以及相关功能分子CD86、TLR4、TLR9的表达水平;ELISA和Griess法检测脾细胞培养上清中IL-10、TNF-α、IFN-γ和NO的分泌水平。日本血吸虫感染增加ECM小鼠体重,降低原虫血症,延长小鼠生存期。混合感染抑制ECM模型小鼠脾脏DC亚群和巨噬细胞的数量,TLR9的表达水平也显著降低。ELISA和Griess检测结果显示混合感染显著降低ECM前炎症细胞因子TNF-α、IFN-γ和NO的表达水平,同时增加Treg的数量和IL-10的表达水平。结果表明,日本血吸虫感染会通过抑制前炎症免疫应答来缓解ECM的发生,从而延长宿主生存期。
英文摘要:
      Severe neurological complications caused by cerebral malaria (CM) are the main death cause in children under 5 years old. Immune response disorder inside the host body is important mechanism that causes the development of CM. The current malaria endemic areas, malaria patients are often accompanied with helminth infections, but the outcome depends on the category of strains, severity, and route of infection. Schistosoma japonicum and ECM models were used to explore the role site of pro inflammatory immune responses in combined infection as well as the correlated immune mechanism. The established combined infection models was adopted to monitor the parasitemia level and survival time in host; DC subpopulations, macrophages, Treg and expression levels of CD86, TLR4 and TLR9 in spleen were determined and tested with FACS. The secretion levels of IL-10, TNF-α, IFN-γ and NO in the spleen cell cultured supernatant were tested and detected with ELISA and Griess method. The results showed that the infection of S. japonicum increased the body weight, reduced parasitemia and prolonged survival time of mice. Combined infection contained the number of splenic DC subgroup, macrophages, and TLR9 expression level was also significantly reduced. ELISA and Griess determination results showed that combined infection significantly reduced the expression level of pro inflammatory cytokines, TNF-α, IFN-γ and NO, the number of Treg and IL-10 level were simultaneously increased. Therefore, the infection of S. japonicum could inhibit the occurrence of ECM through pro inflammatory immune response, accordingly prolonged the survival of the host.
查看全文   查看/发表评论  下载PDF阅读器
关闭