| The research on the function and metabolism of intestines microbial community (IMC) is becoming more and more mature, but the study on the effect of food additive sodium diacetate on human IMC is still rarely reported. In order to explore the potential benefits and harms of sodium diacetate on IMC and human health, this study used mice as models, sodium diacetate was used as the intervener (the intervention dose was 0.3 g/（kg·d）). One week after the intervention, feces of experimental group and control group were collected, and 16S rDNA high-throughput sequencing was used for species identification and abundance detection of the IMC. Data analysis had gained the IMC diversity and abundance ratio and other indices. The results showed that after the intervention of sodium diacetate at the experimental dose, the IMC of mice had significant changes in both species and abundance. with 447 species added and 142 species decreased at the genus level. According to the change of abundance ratio, the most significant changes of 9 species of bacteria, such as Bacteroides, Akkermansia, etc, combined with the prediction of bacterial metabolism function, fatty acid synthesis was significantly up-regulated, and carbohydrate metabolism, amino acid metabolism and lipid metabolism were significantly down-regulated. It was speculated that the high dosage intake of sodium diacetate in may increase the risk of obesity and allergy, chronic inflammation, and gastroenteritis. |
