| This study was aimed at systematically explore and analysis the rumen microbial diversity and gene function of Angus cattle. Six Angus cattle with body weight at 550 kg were selected and used and divided into two groups for omics analysis of rumen fluid samples adopting 16S rRNA high-throughput sequencing technology. The results showed that: A total of 86, 298 high-quality sequences were obtained from the two groups of samples using the Illumina Miseq sequencing platform, clustered into 346 operational taxonomic units (OTU), and identified by taxonomy as 13 phyla, 21 classes, 24 orders, 40 family, and 123 genera. 43.16% of Bacteroidetes and 36.29% of Firmicutes are dominant bacteria. Based on the composition of the genus, they were Prevotella_7 29.28%,Succinivibrionaceae_UCG-001 11.30%, Succiniclasticum 11.10%, Prevotella_1 6.65%, Ruminococcus_1 5.17%, and Succinivibrio 2.75%.It was found by comparison of 16S functional prediction and COG, KEGG metabolic pathway databases that the functions were concentrated in amino acid transport and metabolism, carbohydrate transship and metabolism related genes, which may contain rich proteolysis, transship and metabolism enzyme related genes and a large number of fibers and lignin degrading enzyme genes. Comparison of the CAZymes annotation with the KEGG metabolizing enzyme database showed that the predicted functional genes glycoside hydrolase and glycosyltransferase accounted for a higher proportion; the acetogenic and butyric acid-related enzyme gene abundance was comparatively high. In sum, the rumen of Angus cattle is rich in bacterial population of protein degradation, lignocellulose degradation, and the formation of volatile fatty acid and its enzyme systems, and it laid theoretical foundation to display rumen microbial diversity, discover, and screen new functional enzyme genes. |
