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| 鲍曼不动杆菌碳青霉烯耐药性与生物膜形成及O-甘露糖蛋白相关性研究 |
| Reciprocity in Drug Resistance and Biofilm Formation as well as O-Mannoprotein in Carbapenem Resistant Acinetobacter baumannii |
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| DOI：doi:10.3969/j.issn.1005-7021.2020.02.005 |
| 中文关键词: 鲍曼不动杆菌（Acinetobacter baumannii） 耐药性 生物膜 O-甘露糖蛋白 |
| 英文关键词: Acinetobacter baumannii drug resistance biofilm O-mannoprotein |
| 基金项目:辽宁省自然科学基金项目（20180550594） |
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| 中文摘要: |
| 鲍曼不动杆菌（Acinetobacter baumannii）是引起医院感染的常见致病菌，该细菌不仅容易产生耐药性，而且在人体及无生命物质表面易形成生物膜，临床治疗较为棘手。从临床分离24株鲍曼不动杆菌，药物敏感试验观察这些分离株对常用抗菌药物的敏感性，针对耐碳青霉烯鲍曼不动杆菌，检测是否含有耐药基因碳青霉烯酶基因OXA-23，采用结晶紫染色法观察耐药性与生物膜形成的相关性，并用刀豆蛋白凝集素结合试验及质谱分析耐药性与O-甘露糖蛋白的相关性。结果显示鲍曼不动杆菌耐药性与生物膜形成呈正相关，某些O-甘露糖蛋白表达有利于细菌获得耐药性。 |
| 英文摘要: |
| Acinetobacter baumannii is a common pathogen in hospital infection. It is not only easily to produce drug resistance but also easily to form biofilm in human body as well as on non-biotic surfaces, it is comparatively troublesome in clinical treatment. In this experiment, 24 A. baumannii strains were isolated from clinic. The antibacterial drug susceptibility test was observed to detect the bacterial resistance to common antibiotics if they had carbapenemase gene OXA-23.The reciprocity in drug resistance and biofilm formation was observed adopting crystal violet staining, and mass spectrometrically analyzed the reciprocity in drug resistance and O-mannoprotein adopting sword bean (Canavalia gladiata) agglutinin (ConA) for combining experiment. The results showed that the drug resistance had positive correlation with biofilm formation, and certain O-mannoprotein expression conducive to the bacteria to gain drug resistance. |
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