| Adopting improved and optimized the traditional various isolation media, microbes from tail bottom mud in Fankou lead-zinc mine were preformed the isolation of culturable microbial resources, combined with different molecular biomarker genes to carry out phylogenetic position analysis. The results showed that a total of 41 bacterial strains obtained from Fankou lead-zinc tail bottom mud respectively belonged to 11 orders, 18 families and 18 genera of the phyla of Actinomycetes, Proteobacteria, and Firmicutes; and 8 fungal strains respectively belonged to 2 phyla, 5 orders, 5 families, and 5 genera; 1 alga strain belonged to the genus Galdieria of the phylum of red alga. The improved ISP 2 and improved No.9 medium have shown better isolation effect, and the isolated-and-obtained had fine microbial diversity. This study had laid theoretical basis for studying on the microbial diversity in acid wastewater of mine, and provided material foundation for restoring acid wastewater and ecological control research. |
