林杆菌SK200a-9的重金属铊积累与钾离子的关系

刘姣姣; 刘菊梅; 张笛; 木其尔; 袁浩; 赵吉; 郭永; 太田宽行; 包智华

文章摘要

林杆菌SK200a-9的重金属铊积累与钾离子的关系

Relationship between the Accumulations of Heavy Metal Thallium and Potassium Ions in Alsobacter metallidurans SK200a-9 Thallium and Potassium Ions in Alsobacter metallidurans SK200a-9

DOI：doi:10.3969/j.issn.1005-7021.2019.05.009

中文关键词: Alsobacter metallidurans SK200a-9 铊钾积累

英文关键词: Alsobacter metallidurans SK200a-9 thallium potassium accumulation

基金项目:内蒙古自然科学面上基金项目（2015MS0328）；内蒙古大学高层次人才启动基金项目 (21800-5145144)

作者	单位
刘姣姣	内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
刘菊梅	内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
张笛	内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
木其尔	内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
袁浩	内蒙古大学生态与环境学院，内蒙古呼和浩特 010021
赵吉	1.内蒙古大学生态与环境学院，内蒙古呼和浩特 010021；2.内蒙古大学内蒙古自治区环境污染控制与废物资源化重点实验室, 内蒙古呼和浩特 010021
郭永	日本茨城大学农学部，茨城县阿见町 300 0393
太田宽行	日本茨城大学农学部，茨城县阿见町 300 0393
包智华	1.内蒙古大学生态与环境学院，内蒙古呼和浩特 010021；2.内蒙古大学内蒙古自治区环境污染控制与废物资源化重点实验室, 内蒙古呼和浩特 010021

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中文摘要:

铊（Tl）对敏感细菌的毒性主要由细胞内积累造成，这个过程与钾离子浓度有关。而铊耐性较强的林杆菌（Alsobacter metallidurans）SK200a-9的细胞内铊的积累与钾离子关系尚不清楚。主要分析SK200a-9细胞内Tl⁺积累与K⁺浓度的关系。以SK200a-9为供试菌、Bacillus sp.HK1为对照菌测定添加不同浓度Tl⁺、 K⁺对细胞内Tl⁺积累量的影响，添加1 mmol/L K⁺时静止细胞内Tl⁺的浓度变化。结果表明，两株菌均在Tl⁺浓度1 mmol/L时具有较好的生长，细胞内积累量分别达到最大值255.5和41 ng/mg DW。K⁺浓度恒定时随着Tl⁺浓度的增加，SK200a-9细胞内Tl⁺浓度增加的同时K⁺浓度减少。当Tl⁺：K⁺为1：5时SK200a-9菌体内Tl⁺积累量最高，为419 ng/mg DW。在静止细胞实验中，K⁺的加入使SK200a-9细胞内积累的Tl⁺降低至40%，同时细胞内K⁺浓度直线上升至120 ng/mg DW；而菌株HK1的变化不大。这些结果暗示了Tl⁺使用K⁺转运系统进入到细胞内的可能性。SK200a-9细胞内Tl⁺积累远大于HK1，其耐性机理可能是细胞内积累的Tl⁺通过K⁺作用排出细胞外。

英文摘要:

Toxicity of Thallium (Tl) to sensitive bacteria mainly caused by intracellular accumulation and the accumulation process was related to the concentration of potassium (K) ion. However, the relationship between the accumulation of intracellular T1⁺ and the K⁺ of Alsobacter metallidurans SK200a-9, which is highly tolerant, remain unclear. The main aim of this study was to examine the relationship between the T1⁺ accumulation and K⁺ concentration in cell of A. metallidurans SK200a-9, and studied the thallium tolerance mechanism of the strain. Using A. metallidurans SK200a-9 as the test train and Bacillus sp. HK1 was taken as control to examine the influence of different concentration of K⁺ and Tl⁺ on Tl⁺ accumulation in the cells. The changes of Tl⁺ concentration in stationary cells when 1 mmol/L K⁺ was added. The results showed that both strains had obvious growth at Tl⁺ concentration of 1 mmol/L, and the accumulations in the cells reached 255.5 and 41 ng/mg DW respectively. With the increase of Tl⁺ concentration at constant K⁺ concentration, the increase of Tl⁺ concentration in SK200a-9 cells was accompanied by the decrease of K⁺ concentration. When the ratio of Tl⁺ : K⁺ was at 1：5, the accumulation of Tl⁺ in SK200a-9 was the highest, which was 419 ng/mg DW. In the static cell experiment, the addition of K⁺ reduced the accumulation of Tl⁺ in SK200a 9 cells to 40%, and the concentration of K⁺ in the cells increased to 120 ng/mg DW, while the strain HK1 did not change much. These results implied the possibility of Tl⁺ using K⁺ transport system to enter the cell. Accumulated Tl⁺ in SK200a-9 cells was far higher than that in HK1 cells, and its resistance mechanism may be the Tl⁺ accumulation in the cell was excreted outside the cell by the effect of K⁺.

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