Earthworms are important decomposers of organic matter in soil ecosystem, they can change the formation of microbial community of soil by physical movement and excretion of earthworm feces. In order to study the mutual relationship between microorganisms in earthworm feces and sludge, in this study, Illumina MiSeq high throughput sequencing was used to compare and analyse the bacterial diversity and community structure changes of the sludge and earthworm feces system constructed with Eisenia foetida and sludge established with artificial wetlands matrix adding four carbon sources of glucose, lactose, starch, and cellulose. The results showed that the Chao and ACE indices of sludge were significantly higher than those of earthworm feces, and the Chao and ACE indices of the sludge adding four different carbon sources experimental groups had little difference but higher than those of control group; the Chao and ACE indices of the earthworm feces with glucose, starch, and cellulose were close to those of the control group, but the Chao and ACE indices of the earthworm feces added with lactose resulted in a descent of bacterial diversity. The formation of bacterial structure of earthworm feces and sludge had significant differences, the phyla of Proteobacteria, Bacteroides, Firmicutes, Actinomycetes, Chloroflexi, Acidobacteria, and Gemmatimonadetes were the dominant bacterial phyla in sludge; except for lactose group, the phyla of Proteobacteria, Bacteroides, Firmicutes, Actinomycetes, and Verrumicrobium were the dominant phyla in earthworm feces (among them, the phyla of Proteobacteria, Bacteroides were the absolutely dominant), and the phylum of Mollicutes was detected in earthworm feces lactose group (2.6%), while in other experiment groups had no detected. After adding carbon sources the bacterial communities that could decompose corresponding carbon source increased, for example, Lactococcus piscium in glucose group, Flavobacterium reichenbachii in lactose group, Alkanindiges illinoisensis and Zobellellla taiwanensis in stratch group, and Cellvibrio gandavensis in cellulose group etc. |