文章摘要
甘油影响红曲菌产色素的碳源选择性探究
Exploration on Carbon Source Selectivity of Glycerol Affecting Pigments Production by Monascus spp.
  
DOI：doi:10.3969/j.issn.1005-7021.2019.05.005
中文关键词: 甘油  丛毛红曲菌MS-1  红曲色素  碳源
英文关键词: glycerol  Monascus pilosus MS-1  Monascus pigments (MPs)  carbon source
基金项目:湖北省自然科学基金项目（2017CFB367）；食用野生植物保育与利用湖北省重点实验室开放基金项目（EWPL2017）
作者单位
赵薇 湖北师范大学 生命科学学院，湖北 黄石 435002 
姚钰琪 湖北师范大学 生命科学学院，湖北 黄石 435002 
王爽 湖北师范大学 生命科学学院，湖北 黄石 435002 
石佳 湖北师范大学 生命科学学院，湖北 黄石 435002 
舒思靓 湖北师范大学 生命科学学院，湖北 黄石 435002 
冯艳丽 1.湖北师范大学 食用野生植物保育与利用湖北省重点实验室，湖北 黄石 435002；2.湖北师范大学 生物学国家级 实验教学示范中心，湖北 黄石 435002；3.湖北师范大学 生命科学学院，湖北 黄石 435002 
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中文摘要:
      红曲菌（Monascus spp.）是我国重要的药食同源微生物，红曲色素（Monascus pigments，MPs）是其主要次级代谢产物之一。有研究表明，甘油可促进红曲菌产MPs，但作用机制不明。以丛毛红曲菌（Monascus pilosus）MS-1为实验菌株，考察甘油与葡萄糖或蔗糖复合对红曲菌产MPs的影响。在不含碳源的合成培养基中，将甘油与葡萄糖或蔗糖复合，采用分光光度法和高效液相色谱法等分析MPs的产量和组分、生物量及发酵液pH。当甘油与葡萄糖复合，添加甘油后发酵液pH、生物量无显著变化（P＞0.05），总色价显著降低（P＜0.05）。当2 g/L或40 g/L甘油与蔗糖复合，发酵液pH显著降低而生物量及总色价显著增加（P＜0.05）。当40 g/L甘油与蔗糖复合时，总色价是仅以蔗糖为碳源时的16.5倍，且MPs同系物数量明显增多（P＜0.05）。在合成培养基条件下，甘油促进红曲菌产MPs具有碳源种类的选择性。该结果可为研究甘油影响红曲菌产MPs的作用机制提供参考，为甘油用于MPs生产提供依据。
英文摘要:
      Monascus spp. are important medicine and food homologous microbial resources in China and Monascus pigments (MPs) are a kind of their main secondary metabolites. The studies have showed that glycerol could be enhanced MPs production, however, the mechanism remain unclear. The effects of glycerol compounding with glucose or sucrose on MPs production by Monascus spp. were observed and studied, taken M. pilosus MS-1 as experimental strain. Glycerol compounding with glucose or sucrose in synthetic medium without carbon source, the MPs yields and their components, biomass and pH of fermentation broth were analyzed with spectrophotometry, high performance liquid chromatography etc. No marked changes of pH in fermentation broths and biomass (P>0.05) but significantly decreased total color value were observed when glycerol compounding with glucose (P>0.05). The pH of fermentation broths decreased significantly but the biomass and MPs yield increased significantly when 2 g/L or 40 g/L glycerol compounding with sucrose (P<0.05). The MPs yield was 16.5 times the yield of that sucrose using as the sole carbon source when 40 g/L glycerol compounding with sucrose (P<0.05), and the MPs homologues also increased. There was a selectivity of carbon source sorts when glycerol to improve MPs production under the culture in synthetic medium. The results could lay a foundation for the mechanism study of glycerol on MPs production by Monascus spp., and also provided theoretical support for using glycerol in MPs production.
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