文章摘要
不同浓度Fe2+和Cu2+胁迫对灵芝寡肽转运蛋白基因家族的转录表达水平的影响
Transcriptional Expression Level of Ganoderma lucidum Oligopeptide Transporter (OPT) Protein Gene Family under the Coercion of Different Concentrations of Fe2+ and Cu2+
  
DOI:doi:10.3969/j.issn.1005-7021.2019.05.003
中文关键词: 寡肽转运蛋白  金属  生物量  多糖
英文关键词: oligopeptide transporting protein  metal  biomass  polysaccharide
基金项目:四川省教育厅重点项目(15ZA0013)
作者单位
胡楚霄 四川农业大学 资源学院微生物系四川 成都 611130 
唐恬恬 四川农业大学 资源学院微生物系四川 成都 611130 
师展 四川农业大学 资源学院微生物系四川 成都 611130 
罗霆宇 四川农业大学 资源学院微生物系四川 成都 611130 
向泉桔 四川农业大学 资源学院微生物系四川 成都 611130 
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中文摘要:
      前期研究发现在Fe2+和Cu2+胁迫下,OPT基因转录表达水平会有较大的变化。为进一步探究这两种金属对OPT基因的影响,分析了不同浓度Fe2+和Cu2+胁迫下灵芝寡肽转运蛋白基因家族的转录表达水平。以灵芝荣保1号为实验材料,设置不同浓度梯度(0、50、100、200和400 mg/L)Fe2+和Cu2+进行液体静置培养,分别于15 d和30 d收集样品,测定生物量和多糖含量,利用实时荧光定量PCR技术分析OPT基因的转录表达水平。结果表明,Fe2+对灵芝的生长有一定的促进作用,Cu2+则对其有抑制作用。两种金属在实验用的浓度范围内对于灵芝多糖含量在培养前期表现出抑制作用,后期则有一定的促进作用。除未检测到转录本的3个OPT基因(OPT7、OPT8OPT9),其余OPT基因的转录表达均有差异性。培养前期(15 d),Cu2+胁迫下OPTs基因表达水平较低且差异不明显,Fe2+胁迫下OPTs基因表达水平差异较大;培养后期(30 d),Cu2+胁迫下OPTs基因表达水平较15 d的样品明显增加,且存在差异,Fe2+胁迫下绝大多数OPTs基因均在浓度为200 mg/L下转录表达水平最高。实验证明,不同浓度Fe2+和Cu2+对灵芝的生物量与多糖含量存在不同程度的影响,同时OPT基因在转录水平上也做出了不同的响应,表明其在灵芝适应与吸附外界金属离子的过程起到了重要的作用。
英文摘要:
      Based on previous research, it was found that the transcriptional expression levels of OPT (oligopeptide transporter) protein genes varied largely under the coercion of Fe2+ and Cu2+. In order to further explore the effects of this two metals on OPT genes the present writers analyzed the transcriptional expression levels of OPTs under the coercion of different concentrations of Fe2+ and Cu2+. Using Ganoderma lucidum Rongbao No.1 as the experiment material, the biomass and polysaccharide content were determined when the strain was statically cultured in liquid for 15 d and 30 d under different concentrations (0,50,100, 200 and 400 mg/L) of Fe2+ and Cu2+, then collected samples on 15 d and 30 d respectively, and the transcriptional expression levels of OPTs were analyzed by qRT PCR. The results showed that Fe2+ had a certain promotion on the growth of Rongbao No.1, while Cu2+ inhibited its growth. Both the two metals inhibited the polysaccharides content at early culture stage while promoted it in later culture stage. Expression analysis of the OPTs indicated that, with the exception of three genes (OPT7, OPT8 and OPT9) for which no transcripts were detected, the transcription expression of all other OPTs had differences. At early cultivation stage (15 d), under the coercion of Cu2+ the gene expression level of OPTs was relatively low and had no significant difference; while at the later stage cultivation (30 d) under the coercion of Cu2+ the gene expression levels of OPTs was significantly increased as compared with that of sample at 15 d, and had difference, however, under the coercion of Fe2+ the transcription expression level of the overwhelming majority of OPTs were all at the highest when the Fe2+ concentration at 200 mg/L. Therefore, the biomass and polysaccharides content of G. lucidum were affected at different degrees by different concentrations of Fe2+ and Cu2+, at the same time the transcriptional level of OPTs responded differently, indicating that OPT genes may play important roles during G. lucidum adapting and adsorbing external metal ions.
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