The homology of carbapenem resistant Acinetobacter baumannii(CRAB) was analyzed, its biofilm formation ability was tested, the reciprocity of the gene carrying of blaOXA and biofilm formation was analyzed, and the effective factors of the outbreak of CRAB in the hospital were investigated. A total of 35 non-repeated CRAB strains was collected in the first affiliated hospital of Jiamusi University, and screened the CRAB strains with full-automatic microorganism identification system VITEK-II as well as with in vitro sensitive experiment, multilocus sequence typing (MLST), enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) to analyze their homology, and crystal violet staining to quatitatively check their biofilm formation ability. In the hospital where the authors work, 33 strains of CRAB were ST type 2, one strain ST 671, and one strain ST 1199 was newly discovered MLST subtype. According to ERIC they could be divided into A, B, C, and D types, A type 9 strains, B type 24 strains, C type one strain, and D type one strain. And there was significant statistic differences of the biofilm formation ability of CRAB and carbapenem sensitive Acinetobacter baumannii(CSAB) groups (0.330±0.258, 0.534±0.402, P<0.05); and there was no significant reciprocity between gene carrying of blaOXA-23, blaOXA-51, and blaOXA-24 gene and CRAB biofilm formation ability (P>0.05). And the biofilm formation ability was negatively correlated with carbapenem resistance obtainment. The resolution of ERIC-PCR was relatively strong, and it had a certain correlation with MLST. ST2 type was the main epidemic strain in the hospital. The high risk factors of the patients and the sterilization awareness of the doctors and nurses may be related to the outbreak of CRAB. |