文章摘要
解脂亚罗酵母菌体外降甘油三酯降胆固醇效果研究
Effect of Yarrowia lipolytica on Triglyceride and Cholesterol Reduction in vitro
  
DOI:doi:10.3969/j.issn.1005-7021.2019.04.010
中文关键词: 酵母菌  甘油三酯  胆固醇  毒性试验  26S rDNA
英文关键词: yeast strain  triglyceride  cholesterol  toxicity test  26S rDNA
基金项目:中央高校基本科研专项资金项目(31920170034);国家民委科研项目(14XBZ015);甘肃省农牧厅攻关项目(GNSW-2007-09)
作者单位
吴慧昊 西北民族大学 实验中心甘肃 兰州 730030 
牛锋 西北民族大学 生命科学与工程学院甘肃 兰州 730030 
徐红伟 西北民族大学 实验中心甘肃 兰州 730030 
钟琦 湖北省当阳市动物疫病预防控制中心湖北 当阳 444100 
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中文摘要:
      筛选出高效降三酰甘油降胆固醇菌株,为新型药物、食品的制备提供优良菌株。从样品中筛选分离出酵母菌株,并测定菌株降三酰甘油能力,采用磷硫铁比色法测定菌株降胆固醇能力,通过耐酸、耐胆盐及毒性实验研究菌株各项性能,通过生理生化及26S rDNA法对菌株进行鉴定。结果显示,试验筛选出1株高效降脂降胆固醇酵母菌EAM-ZT003T,降三酰甘油能力高达80.2%,菌株具有很好的耐酸、耐胆盐能力,为期42 d的小鼠毒性试验发现,小鼠未出现异常体征,未发现异常死亡,且解剖观察各器官一切正常,菌株经26S rDNA鉴定为解脂亚罗酵母菌(Yarrowia lipolytica)。
英文摘要:
      Triglyceride and cholesterol-lowering excellent strains was screened in order to prepare new drugs and food. Isolation and purification of yeast strains from samples and test for its triglyceride degradation ability of the target strains, and the cholesterol lowering ability was determined adopted phosphorus-sulfur-iron colorimetry. The screened strains were further investigated for their tolerance to acid and bile salt, and toxicity test and other performances. Physiological, biochemical tests, and 26S rDNA method were used to identify the strains. The results showed that the experiment had screened a triglyceride and cholesterol degradable strain EAM-ZT003, particularly the triglyceride degradation ability was as high as 80.2%. And the results showed the strain had fine tolerance to acid and bile salt. And it was found that in 42 d of toxicity test in mice and revealed no abnormal pathological sign and exceptional death, and all the dissected organs of mice were observed to be normal. The strain EAM-ZT003T was identified as Yarrowia lipolytica adopting 26S rDNA.
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