| 探讨柯萨奇病毒（coxsackievirus, CVB）对人类少突胶质细胞（oligodendrocyte, OL）和博尔纳病病毒（Borna disease virus, BDV）持续感染的OL细胞（OL/BDV）中I型干扰素（interferon, IFN）、microRNA-155（miR-155）表达以及干扰素调节因子（interferon regulatory factor, IRF）7细胞定位的影响。CVB感染OL细胞和OL/BDV细胞0、2、4、6、12和24 h，qPCR检测I型IFN mRNA和miR-155表达水平。OL细胞和OL/BDV细胞分别转染IRF7-EGFP质粒，CVB感染4 h，观察IRF7细胞定位情况。CVB感染OL细胞2、4、12和24 h，IFN-α mRNA和miR-155表达水平显著增加，IFN-β mRNA表达水平在4、12和24 h显著增加；CVB感染OL/BDV细胞IFN-α和IFN-β mRNA表达水平除0 h均显著增加，但miR 155表达仅在12和24 h显著增加。CVB感染OL细胞和OL/BDV细胞4 h，IRF7绿色荧光蛋白由细胞浆转移至细胞核内。综上所述，CVB可诱导OL细胞和OL/BDV细胞I型IFN、miR-155的表达，促进IRF7入核，从而激活I型IFN信号转导途径。 |
| Effects of coxsackievirus (CVB) on the expression of type I interferon (IFN) and microRNA-155 (miR-155) as well as the localization of interferon regulatory factor (IRF) 7 in human oligodendrocyte (OL) and Borna disease virus (BDV) infected OL cells (OL/BDV) were investigated. After the CVB infection, the expression of type I IFN mRNA and miR-155 in OL cells and OL/BDV cells were detected by qPCR on 0, 2, 4, 6, 12 and 24 h and observed the localization of IRF7 cells. OL cells and OL/BDV cells were respectively transfected to IRF7 EGFP plasmid and infected with CVB for 4 h and observed the cell localization. The expression of IFN-α mRNA and miR-155 significantly increased at 2, 4, 12 and 24 h, and IFN-β mRNA significantly increased at 4, 12 and 24 h in OL cells. The expression of IFN-α and IFN-β mRNA in OL/BDV cells infected with CVB all increased significantly at most time points detected except 0 h, however, miR 155 increased only at 12 and 24 h. In OL cells and OL/BDV cells infected with CVB for 4 h, IRF7 green fluorescent protein was translocated from cytoplasm into nucleus. In summary of all above, CVB could induce the expression of type I IFN and miR-155 and promote the entrance of IRF7 into nucleus, thus activated the type I IFN signal transduction pathway. |
