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| 氢氧化细菌SDW-16的分离鉴定及促生特性 |
| Isolation, Identification and Growth-Promoting Properties of Hydrogen Oxidizing Bacterium SDW-16 |
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| DOI：doi:10.3969/j.issn.1005-7021.2019.04.007 |
| 中文关键词: 氢氧化细菌 铁载体 IAA ACC脱氨酶 16S rDNA |
| 英文关键词: hydrogen oxidizing bacterium iron carrier IAA ACC deaminase 16S rDNA |
| 基金项目:国家自然科学基金项目(41571243)；西安医学院配套基金项目（2017PT14）；西安医学院国家基金培育项目（2017GJFY16） |
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| 中文摘要: |
| 利用气体循环培养体系从沙打旺根际土壤分离得到1株氢氧化细菌SDW-16（GenBank登录号：KF835389），16S rDNA序列分析和生理生化特征鉴定其为荧光假单胞菌（Pseudomonas fluorescens）。菌株对植物促生机制的初步研究表明菌株SDW-16除具有铁载体分泌能力外，还具有产IAA和ACC脱氨酶活性，其中产IAA量为（21.62±0.30） μg/mL，ACC脱氨酶活力高达（8 372.17±805.43） nmol/（mg·h）。菌株SDW-16具有多项促生能力且均高于其他菌株，说明菌株SDW-16有较高的促生特性，同时也初步证明了氢氧化细菌的促生机制。 |
| 英文摘要: |
| A hydrogen oxidizing bacterium strain SDW-16 (GenBank accession number: KF835389) was isolated from the rhizosphere soil of Astragalus adsurgens Pall using the gas circulation culture system. The bacterium SDW-16 was identified as Pseudomonas fluorescens by 16S rDNA sequence analysis and physiological and biochemical characteristics. Preliminary studies on the plant growth promoting mechanism showed that the strain SDW-16 had the ability to produce IAA and ACC deaminase in addition to the iron carrier secretion capacity, and the production of IAA was （21.62±0.30） μg/mL, ACC deaminase activity was up to （8 372.17±805.43） nmol/（mg·h）. The strain SDW-16 had a number of growth promoting abilities and was higher than other strains, indicating that the strain SDW-16 had higher growth promoting property, and also preliminarily have proved the promoting mechanism of the hydrogen oxidizing bacteria. |
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