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| 转IE2基因荷瘤小鼠中ATF5功能影响的研究 |
| Effects of ATF5 Function on IE2 Transgenic Mice Bearing Tumor |
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| DOI：doi:10.3969/j.issn.1005-7021.2019.02.011 |
| 中文关键词: 人巨细胞病毒 IE86 鼠胶质瘤细胞 转录激活因子5(ATF5) 动物模型 |
| 英文关键词: human cytomegalovirus IE86 mouse glioma cell ATF5 animal model |
| 基金项目:国家自然科学基金面上项目（81471958）；青岛市博士后应用研究项目（2015160） |
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| 中文摘要: |
| 利用转IE2基因小鼠荷瘤的方法，对肿瘤组织中ATF5转录激活因子进行定性和定量的检测，从而探究巨细胞病毒 (HCMV) 即刻早期蛋白IE86对ATF5功能的影响。通过鼠胶质瘤细胞GL261对转基因鼠进行荷瘤，采用RT-PCR和Western blot的方法，对荷瘤鼠的肿瘤组织中转录因子ATF5的表达量进行检测。采用RT-PCR和Western blot的方法检测荷瘤鼠肿瘤组织中转录因子ATF5的表达，结果显示,转IE2基因小鼠体内ATF5表达量较正常小鼠ATF5表达量高。ATF5在转IE2基因荷瘤鼠中呈现高表达状态，ATF5是一个与凋亡密切相关的转录因子，在凋亡、分化及发育等生理过程中发挥着重要作用。 |
| 英文摘要: |
| Qualitative and quantitative detections of ATF5 activated transcription factors in tumor tissue were carried out using method of transfection IE2 mouse tumor to explore the effect of HCMV immediate early protein IE86 on ATF5 function. Transfected mice were treated with GL261 mouse glioma cells. The expression of transcription factor ATF5 in the tumor tissues of tumor bearing mice was detected by RT-PCR and Western blot methods. RT-PCR and Western-blotting were used to detect the expression of transcription factor ATF5 in the tumor tissue of tumor-bearing mice. The experimental results showed that the expression of ATF5 was higher in IE2 transgenic mice than in normal mice. ATF5 assumed high expression condition in IE2 transgenic mice, and ATF5 was a transcription factor closely related to apoptosis and played important roles in apoptosis, differentiation and development and other physiological processes. |
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