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| 硫胺素及溶氧对解脂亚洛酵母联产酮酸的影响 |
| Effects of Thiamine and Dissolved Oxygen on Co-Production of α-Ketoglutaric Acid and Pyruvic Acid by Yarrowia lipolytica |
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| DOI：doi:10.3969/j.issn.1005-7021.2019.02.005 |
| 中文关键词: 解脂亚洛酵母 联产 α-酮戊二酸 丙酮酸 |
| 英文关键词: Yarrowia lipolytica co-production α-ketoglutaric acid pyruvic acid |
| 基金项目:国家自然科学基金重大项目(21390204) |
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| 中文摘要: |
| α-酮戊二酸和丙酮酸是重要的酮酸，广泛应用于食品、医药等领域。以本研究室在前期的工作中诱变选育的Yarrowia lipolytica WSH-Z06 C3为出发菌株，高效联产α-酮戊二酸和丙酮酸。在摇瓶水平上初步确定了最佳氮源浓度以及接种密度。在此基础上，重点考察了在15 L发酵罐上硫胺素浓度以及溶氧控制参数对酮酸联产的影响。结果显示，硫胺素最佳浓度为0.2 μg/L，α-酮戊二酸和丙酮酸分别高达24.5、53.7 g/L。与未优化的对照相比，酮酸总产量和丙酮酸总转化率均提高了57.9%。溶氧水平控制在50%，发酵96 h酮酸总产量高达53.2 g/L。通过两阶段溶氧调控，酮酸总产量高达64.7 g/L，比未调控前提高了21.2%。通过硫胺素与溶氧水平的优化，显著提高了酮酸的产量，进一步为酮酸的工业化生产提供参考。 |
| 英文摘要: |
| α-Ketoglutaric acid and pyruvic acid are important keto-acids widely used in food, pharmaceutical and other industries. In this study, Yarrowia lipolytica WSH-Z06 C3 that has been mutagenized and bred in previous work as starting strain to co-produce α-ketoglutaric acid and pyruvic acid high-effectively. The optimum nitrogen source concentrations and inoculation density were confirmed initially in shaker flasks. Based on these results, effects of the thiamine concentration and dissolved oxygen control parameters on the co-production of keto acids were emphatically investigated in 15 L fermentor. The results showed that the titer of α-ketoglutaric acid and pyruvic acid were 24.5 g/L and 53.7 g/L when the optimum concentration of thiamine concentration was 0.2 g/L. Both the total production of keto acid and the yield of keto acids on glycerol increased by 57.9% higher than that of the control before the optimization. The total output of keto acids was as high as 53.2 g/L when dissolved oxygen level was controlled at 50%. Two-stage control of dissolved level was investigated, the total output of keto acids was as high as 64.7 g/L and increased by 21.2% as compared with that before regulated. The yield of keto acids can be significantly improved by optimizing the dissolved oxygen and thiamine concentration, which will further lay a foundation for the industrialized production of keto acid. |
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