| A detection method using loop mediated isothermal amplification (LAMP) for fast, accurate, and convenient detection of feline herpes virus I (FHV-1) was established. According to TK gene of FHV-I in Genebank primers were designed, optimized the reaction conditions the amplification effects were observed and analyzed through agarose gel electrophoresis and SYBR Green staining. This method had fine specificity, the sensitivity detection experiment suggested that the detection method could detect the template at the minimum 101 copies/μL, being 10 times sensitivity of PCR detection method. Four different amplification reaction instruments metal bath, oven, constant temperature water bath, and PCR instrument all could meet the requirement of LAMP, and showed amplification trapezoid bands. The establishment of LAMP detection for FHV-I, with its amplification primers of high specificity, the technical requirements for detection instruments, reaction conditions, as well as personnel was fairly ample, the time used from the starting of the amplification through the results of the realization of SYBR Green I to visibly unscramble was less than one hour, it really realized the goal of the detection of FHV-I with rapid, accurate, and simple. |
