| Aerobic plate counting was used to study bacterial diversity and community structure in paddy fields of rice oilseed double serial multiple cropping with no-tillages (NT) styles and ploughing tillage (PT). The plate counting (PC) was adopted to count the quantity of culturable bacteria and actinomycetes; and analyzed the specific diversity and community structure with Miseq sequencing analyses. The results showed that the total colony quantity of culturable in NT was higher than that of PT by 10% with comparison of three sample points, and a total of 224 563 valid sequences was obtained in uncultured soil sample, among them NT 114 433, PT 110 220. The index analysis showed that the relative abundance of PT was slightly lower than that of NT, and the community diversity was slightly lower than that of NT. The bacterial species in the sample covered 35 phylums, 269 families, and 373 genera. Although different from the level of classification, sample species relative abundance of PT, NT had no significant difference, but the sequences of the largest phylum of Proteobacteria bacteria in NT bacterial phylum and the species of acidobacteria phylum that remained for further study on acidobacteria species sequence is more than PT, sequences of Anaerolineaceae, unclassified Nitrospiraceae, and Nitromonaceae in NT were more than those in PT, in addition, the species of unknown and uncultured families, genera level were more than those in PT, it was believed according to specific characteristics they may play important role on nitrogen fixation, cellulose degradation, and changing the physicochemical properties in maintaining soil fertility of no tillage. |
