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| 酿酒酵母L610利用菊糖生产乙醇发酵条件的研究 |
| Fermentation Conditions of Ethanol Production Using Inulin by Saccharomyces cerevisiae L610 |
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| DOI：doi:10.3969/j.issn.1005-7021.2018.04.006 |
| 中文关键词: 乙醇 菊糖 酿酒酵母 发酵条件 |
| 英文关键词: ethanol inulin Saccharomyces cerevisiae fermentation conditions |
| 基金项目:国家自然科学基金项目（31671796）；辽宁省自然科学基金项目（201602059）；辽宁省高等学校杰出青年学者成长计划（LJQ2015009） |
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| 中文摘要: |
| 以1株能够直接利用菊糖产乙醇的酿酒酵母L610为出发菌株，对其利用菊糖生产乙醇的发酵条件进行了一系列研究。结果表明，L610最适乙醇发酵温度为37 ℃，且40 ℃高温发酵对其产乙醇能力无显著影响；L610对酸性发酵环境有良好的耐受性，当发酵液pH值降至3.5时，其糖醇转化率及乙醇产量仍保持较高水平；以0.025~0.10 vvm的通气量通气12 h有利于L610发酵菊糖产乙醇；L610对350 g/L的高浓度菊糖有良好的转化率，乙醇浓度和生产强度分别达到129 g/L和1.35 g/（L·h）；当直接以300 g/L菊芋粗粉为唯一底物进行发酵时，L610发酵产乙醇浓度达到89.6 g/L，为理论产量的78.1%。本研究所取得的成果为酿酒酵母一步法发酵菊芋生产乙醇的工业化发展提供参考。 |
| 英文摘要: |
| Different fermentation conditions of ethanol production by a capable directly inulin using Saccharomyces cerevisiae strain L610 as a starter were carried out a serial studies. The results showed as follows: the optimum ethanol production temperature by strain L610 was 37 ℃, whereas high temperature at 40 ℃ had no significant effects on the ethanol fermentation capability of L610; L610 showed a good tolerance against acidic circumstances, when pH of fermentation broth declined to 3.5 the sugar ethanol conversion rate could even remain at a fairly high level, when the fermentation tank was aerated at 0.025—0.10 vvm for 12 h, it was conducive to L610 to ferment inulin to produce ethanol, the concentration and production intensity reached up to 129 g/L and 1.35 g/（L·h） respectively, when crude Jerusalem artichoke (Helianthus tuberosus) powder was directly used as the only substratum at 350 g/L, L610 could produce ethanol at concentration as high as 89.6 g/L, which was 78.1% of the theoretical ethanol yield. The outcome achieved in this work had laid a foundation for one step fermentation of industrial ethanol production using Jerusalem artichoke by S. cerevisiae strains. |
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