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| 诺如病毒GII.6 P粒子原核表达与纯化 |
| Prokaryotic Expression and Purification of Norovirus GII.6 P Particle |
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| DOI：doi:10.3969/j.issn.1005-7021.2018.02.013 |
| 中文关键词: 诺如病毒（Noroviruses,Novs） P粒子 原核表达 纯化 |
| 英文关键词: Norovirus p particle prokaryotic expression purification |
| 基金项目:国家自然科学基金项目（31601570） |
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| 中文摘要: |
| 原核表达的诺如病毒（Noroviruses , NoVs）衣壳蛋白亚基P粒子与NoV有相同的抗原类型，可以代替NoV在体外进行结合，这对于研究该病毒与宿主和环境载体结合的相关机理有重大意义。将GII.6 P粒子基因克隆到表达载体pGEX-4T-1上并同时在大肠埃希菌BL21(DE3)、Rosetta和OrigamiB菌株中进一步优化原核表达体系。结果表明重组质粒pGEX-4T-1-GII.6 P在22 ℃、2×10-4mol/L IPTG诱导22 h后表达量最高，且BL21菌株的可溶性表达水平高于其他菌株。随后，在亲和层析的基础上结合阴离子交换以及凝胶过滤层析对表达产物进行纯化，最终获得高纯度GII.6 P粒子。 |
| 英文摘要: |
| The P particles, subunits of Norovirus (NoV) capsid protein, show the same antigen type as NoVs and are capable of work as an alternative in vitro binding study, which is of great significance to study the mechanism of the binding of NoVs with their hosts. Hence, in this study, the NoV GII.6 P gene was cloned into pGEX-4T-1 plasmid, then the recombinant GII.6 P was expressed simultaneously in different strains of E. coli, BL21(DE3), Rosetta and OrigamiB for further optimization of the expression system. The results showed that the expression level of recombinant plasmid pGEX-4T-1 GIL.6P after 22 ℃ and induced for 22 h at concentration of 2×10-4 mol/L IPTG reached the highest. Subsequently, the soluble expression level of BL21 strain was higher than that of other strains. Soon after, the GII.6 P particles were purified based on affinity chromatography combined with anion exchange and gel filtration chromatography, finally obtained high purity of GII.6 P particles. |
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