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| 草酸对铜绿假单胞菌NY3降解烃的作用研究 |
| Effects of Oxalic Acid on Hydrocarbon Degradation by Pseudomonas aeruginosa NY3 |
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| DOI：doi:10.3969/j.issn.1005-7021.2018.02.011 |
| 中文关键词: 铜绿假单胞菌NY3 草酸 PCA 十四烷 生物降解 |
| 英文关键词: Pseudomonas aeruginosa NY3 oxalic acid phenazine-1-carboxylic acid (PCA) tetradecane biodegradation |
| 基金项目:国家自然科学基金项目(51278405); 国家科技支撑计划项目（2014BAC13B06） |
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| 中文摘要: |
| 研究了草酸对NY3菌降解烷烃的影响作用。研究发现，草酸能促进菌体降解十四烷，且最佳投加浓度在1 g/L左右。投加草酸8 h内对十四烷去除率最大，可提高约15.2%。草酸促进降解主要是其改变菌体胞外液分泌成分。紫外光谱结果表明，共存草酸使胞外液中吩嗪-羧酸PCA分泌量大大提高。从气相和液质测定结果看，NY3菌代谢体系中，胞外液中PCA分泌量与十四烷的同步降解率呈正相关。体外实验表明，将试剂级PCA投加在NY3菌以“十六烷+草酸”为碳源生长的胞外液中，发现PCA投加量与其对十四烷的降解效果也呈正相关。与未投加PCA相比，PCA投加2 μmol/L，12 h内胞外液对十四烷的去除率提高了约13.1%。结果表明，草酸能促进NY3菌降解十四烷主要是由于其可使菌体胞外液中PCA的分泌量大大提高。本文研究了共存草酸在铜绿假单胞菌NY3降解烃类污染物过程中的作用及其作用机理，为后续优化设计铜绿假单胞菌NY3处理实际烃类污染物的应用提供参考。 |
| 英文摘要: |
| The effects of oxalic acid on hydrocarbons degradation by Pseudomonas aeruginosa NY3 were studied. It was found in the study that oxalic acid could promote the degradation of tetradecane by P. aeruginosa NY3, and the optimum adding concentration was about 1 g/L. The addition of oxalic acid could increase the removal rate of tetradecane by utmost 15.2% within 8 hours. It was found in the study that promotion degradation of oxalic acid mainly changed the secretion composition of extracellular liquid. The results of UV spectrum showed that coexistent oxalic acid made the secretion of phenazine-1-carboxylic acid (PCA) in extracellular fluid greatly increased. From the testing results by gas chromatography and the Time of Flight Mass Spectrometer (LCMS-IT-TOF), between the PCA secretion in extracellular liquid and synchronous degradation rate of tetradecane assumed a positive correlationship in metabolism system of strain NY3. Experiments in vitro showed that when the reagent grade PCA was added to the growth extracellular fluid of strain NY3 with ″hexadecane + oxalic acid″ as carbon source, it was found that the addition of PCA was positively correlated to the degradation effects of tetradecane. As compared the one that not adding with PCA, the addition of PCA at 2 μmol/L, removal rate of tetradecane increased by about 13.1% within 12 hours. The results of this study showed that oxalic acid could promote the degradation of tetradecane by strain NY3 mainly due to great increment of the PCA secretion in the extracellular fluid. This paper had studied that the effect and effective mechanism of coexistent oxalic acid during the hydrocarbon pollutant degradation by P. aeruginosa NY3, and provided important theoretical reference for the subsequent optimization design of the application of P. aeruginosa NY3 to the treatment of actual hydrocarbon contaminants. |
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