|
| Mg2+对米曲霉HMP-F28所产α-，β-CPA的调控作用 |
| Effect of Mg2+ on the Production of α-CPA, β-CPA in Aspergillus oryzae HMP-F28 |
| |
| DOI：doi:10.3969/j.issn.1005-7021.2018.02.002 |
| 中文关键词: 环匹阿尼酸 IAA拮抗剂 米曲霉（Aspergillus oryzae）HMP-F28 SPE-LC-MS |
| 英文关键词: cyclopiazonic acid IAA antagonist Aspergillus oryzae HMP-F28 SPE-LC-MS |
| 基金项目:国家自然科学基金项目（31471814） |
|
| 摘要点击次数: 743 |
| 全文下载次数: 61 |
| 中文摘要: |
| 在米曲霉HMP-F28所产CPA(环匹阿尼酸)类化合物中，β-CPA是α-CPA的生物合成前体化合物，因此β-CPA的含量很低，而α-CPA是主要代谢产物。在研究HMP-F28所产CPA类代谢产物的调控过程中，采用SPE-LC-MS方法建立了一个对HMP-F28所产CPA类代谢物的快速定性分析方法。在此基础上，发现不同浓度的Mg2+可调控α-CPA和β-CPA相对含量，高浓度Mg2+（320 mmol/L）可使菌株显著提高生物合成中间体β-CPA的产量。由于β-CPA在结构上较α-CPA与IAA拮抗剂hypaphorine更类似，因此本研究为进一步研究其作为IAA拮抗剂诱导植物免疫反应的活性提供参考。 |
| 英文摘要: |
| Among CPAs (cyclopiazonic acids) produced by Aspergillus oryzae HMP-F28, β-CPA is a biosynthetic precursor of α-CPA, in consequence the content of β-CPA is very low, and α-CPA is the major metabolite. During the regulation and control process of CPAs production by HMP-F28, a SPE-LC-MS method was adopted to establish a rapid qualitative analysis for CPAs metabolite produced by HMP-F28. It was found based on these that different concentration of Mg2+ could regulate and control the relative content of α-CPA and β-CPA, high concentration of Mg2+(320 mmol/L) could significantly increase the output of β-CPA. Since the structure of β-CPA is more similar to IAA antagonist hypaphorin, therefore, this study has laid a foundation to further study on β-CPA as IAA antagonist to induce the activity of plant immune responses. |
|
查看全文
查看/发表评论 下载PDF阅读器 |
| 关闭 |
