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| 海洋来源塔宾曲霉LWG-42菌株的鉴定及其抗氧化活性化合物的分离 |
| Identification of Marine Derived Aspergillus tubingensis LWG-42 and Isolation of Its Antioxidant Active Compounds |
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| DOI：doi:10.3969/j.issn.1005-7021.2017.06.005 |
| 中文关键词: 海洋真菌 塔宾曲霉 抗氧化活性 菌株鉴定 活性追踪分离 |
| 英文关键词: marine fungus Aspergillus tubingensis antioxidant activity strain identification bioassay guided fractionation |
| 基金项目:江苏省自然科学基金项目(BK20151283)；江苏省海洋药物分子筛选重点实验室开放基金项目(2015HYB10)；江苏省海洋生物技术重点实验室开放基金项目(2014HS006)； 连云港市科技项目(CG1612) |
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| 中文摘要: |
| 鉴定1株具有抗氧化活性的海洋真菌LWG 42 菌株，分离鉴定其抗氧化活性化合物。通过形态学特征和ITS 序列分析对菌株LWG 42 进行鉴定；利用DPPH 自由基清除试验测定抗氧化活性，通过溶剂萃取和柱层析分离LWG 42 菌株产生的抗氧化活性化合物；通过化合物的波谱特征鉴定其结构。菌株LWG 42 被鉴定为塔宾曲霉 (〖WTBX〗Aspergillus tubingensis〖WTBZ〗)；从其发酵产物中分离得到2个化合物aurasperone A 和 aurasperone B，aurasperone A、B清除DPPH 自由基的EC50 值分别为0.18 和0.11 mg/mL。实验结果为进一步开发这两个化合物在药品、化妆品和食品工业中的应用提供了参考。 |
| 英文摘要: |
| A marine fungal strain LWG-42 with antioxidant activity and was isolated, and its antioxidant compounds were isolated and identified. The strain LWG-42 was identified based on its morphological features and internal transcribed spacer (ITS) sequence analysis. The DPPH (1,1-diphenyl-2 picryhydrazyl) free radical scavenging assay was used to determine its antioxidant activity, and the active compounds produced by strain LWG 42 were bioassay guided isolated and purified by solvent extraction and column chromatography from the culture broth of the strain LWG 42. The structure of the compounds was identified based on spectral characteristics′. The results showed that LWG 42 was identified as Aspergillus tubingensis. Two compounds from fermented products were aurasperone A and aurasperone B isolated and obtained. And the DPPH radical scavenging activities EC50 values of aurasperone A and B were respectively at 0.18 and 0.11 mg/mL. The experimental results provided the foundations to further develop the applications of aurasperone A and B in pharmacy, cosmetic, and food industries. |
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